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Preheating accelerates mitogen-activated protein (MAP) kinase inactivation post-heat shock via a heat shock protein 70-mediated increase in phosphorylated MAP kinase phosphatase-1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kyoung-Hee | - |
dc.contributor.author | Lee, Choon-Taek | - |
dc.contributor.author | Kim, Young Whan | - |
dc.contributor.author | Han, Sung Koo | - |
dc.contributor.author | Shim, Young-Soo | - |
dc.contributor.author | Yoo, Chul-Gyu | - |
dc.date.accessioned | 2010-01-12T04:38:09Z | - |
dc.date.available | 2010-01-12T04:38:09Z | - |
dc.date.issued | 2005-01-29 | - |
dc.identifier.citation | J Biol Chem. 2005 Apr 1;280(13):13179-86. Epub 2005 Jan 26. | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15677475 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29655 | - |
dc.description.abstract | Heat shock (HS) activates mitogen-activated protein (MAP) kinases. Although prior exposure to nonlethal HS makes cells refractory to the lethal effect of a subsequent HS, it is unclear whether this also occurs in MAP kinase activation. This study was undertaken to evaluate the effect of a heat pretreatment on MAP kinase activation by a subsequent HS and to elucidate its possible mechanism. Preheating did not make BEAS-2B cells refractory to extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK) activation by a second HS but accelerated their inactivation after HS. The rapid inactivation of ERK and JNK was dependent on de novo protein synthesis and associated with the up-regulation of heat shock protein 70 (HSP70). Moreover, the inhibition of phosphatase activity reversed this rapid inactivation. MAP kinase phosphatase-1 (MKP-1) expression was increased by HS, and the presence of its phosphorylated form (p-MKP-1) correlated with the observed rapid ERK and JNK inactivation. Blocking induction of p-MKP-1 with antisense MKP-1 oligonucleotides suppressed the rapid inactivation of ERK and JNK in preheated cells. HSP70 overexpression caused the early phosphorylation of MKP-1. Moreover, MKP-1 phosphorylation and the rapid inactivation of ERK were inhibited by blocking HSP70 induction in preheated cells. In addition, MKP-1 was insolubilized by HS, and HSP70 associated physically with MKP-1, suggesting that a chaperone effect of HSP70 might have caused the early phosphorylation of MKP-1. These results indicate that preheating accelerated MAP kinase inactivation after a second HS and that this is related to a HSP70-mediated increase in p-MKP-1. | en |
dc.language.iso | en | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.subject | Adenoviridae/genetics | en |
dc.subject | Animals | en |
dc.subject | Blotting, Western | en |
dc.subject | Bronchi/cytology | en |
dc.subject | Cell Cycle Proteins/*antagonists & inhibitors/*metabolism | en |
dc.subject | Cells, Cultured | en |
dc.subject | Dual Specificity Phosphatase 1 | en |
dc.subject | Enzyme Inhibitors/pharmacology | en |
dc.subject | Epithelial Cells/cytology | en |
dc.subject | HSP70 Heat-Shock Proteins/*metabolism | en |
dc.subject | Hot Temperature | en |
dc.subject | Humans | en |
dc.subject | Immediate-Early Proteins/*antagonists & inhibitors/*metabolism | en |
dc.subject | JNK Mitogen-Activated Protein Kinases/metabolism | en |
dc.subject | MAP Kinase Kinase 4 | en |
dc.subject | Mice | en |
dc.subject | Mitogen-Activated Protein Kinase Kinases/metabolism | en |
dc.subject | NIH 3T3 Cells | en |
dc.subject | Oligonucleotides/chemistry | en |
dc.subject | Oligonucleotides, Antisense/chemistry | en |
dc.subject | Phosphoprotein Phosphatases/*antagonists & inhibitors/*metabolism | en |
dc.subject | Phosphorylation | en |
dc.subject | Protein Binding | en |
dc.subject | Protein Phosphatase 1 | en |
dc.subject | Protein Tyrosine Phosphatases/*antagonists & inhibitors/*metabolism | en |
dc.subject | Time Factors | en |
dc.subject | Up-Regulation | en |
dc.subject | MAP Kinase Signaling System | - |
dc.title | Preheating accelerates mitogen-activated protein (MAP) kinase inactivation post-heat shock via a heat shock protein 70-mediated increase in phosphorylated MAP kinase phosphatase-1 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이경희 | - |
dc.contributor.AlternativeAuthor | 이춘택 | - |
dc.contributor.AlternativeAuthor | 김영환 | - |
dc.contributor.AlternativeAuthor | 한성구 | - |
dc.contributor.AlternativeAuthor | 심영수 | - |
dc.contributor.AlternativeAuthor | 유철규 | - |
dc.identifier.doi | 10.1074/jbc.M410059200 | - |
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