S-Space College of Medicine/School of Medicine (의과대학/대학원) Pediatrics (소아과학전공) Journal Papers (저널논문_소아과학전공)
Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha
- Issue Date
- World J Gastroenterol. 2007 Apr 7;13(13):1962-5.
- Adenocarcinoma/metabolism/pathology/physiopathology ; Caco-2 Cells ; Colonic Neoplasms/metabolism/pathology/physiopathology ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Humans ; I-kappa B Proteins/metabolism ; Interleukin-8/*metabolism ; Intestinal Absorption/drug effects/*physiology ; Intestinal Mucosa/drug effects/*physiology ; Membrane Potentials/drug effects/physiology ; Probiotics/*pharmacology ; Tumor Necrosis Factor-alpha/*pharmacology ; Lactobacillus plantarum
- AIM: To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells. METHODS: Caco-2 cells were incubated with TNF-alpha alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IkappaB-alpha. RESULTS: A TNF-alpha-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-alpha-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IkappaB-alpha in TNF-alpha-treated Caco-2 cells. CONCLUSION: Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-alpha is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.
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