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Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Byung Joo | - |
dc.contributor.author | Jun, Jae Yeol | - |
dc.contributor.author | So, Insuk | - |
dc.contributor.author | Kim, Ki Whan | - |
dc.date.accessioned | 2010-01-18T04:00:01Z | - |
dc.date.available | 2010-01-18T04:00:01Z | - |
dc.date.issued | 2006-03-08 | - |
dc.identifier.citation | World J Gastroenterol. 2006 Feb 7;12(5):796-9. | en |
dc.identifier.issn | 1007-9327 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16521198 | - |
dc.identifier.uri | https://hdl.handle.net/10371/36239 | - |
dc.description.abstract | AIM: Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal. METHODS: Enzymatic digestions were used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs. RESULTS: Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner. Clonazepam from 20 to 60 micromol/L and CGP37157 from 10 to 30 micromol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50s of clonazepam and CGP37157 were 37.1 and 18.2 micromol/L, respectively. The addition of 20 micromol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs. CONCLUSION: These results suggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity. | en |
dc.language.iso | en | en |
dc.publisher | WJG Press | en |
dc.subject | Animals | en |
dc.subject | Biological Clocks/drug effects/*physiology | en |
dc.subject | Clonazepam/analogs & derivatives/pharmacology | en |
dc.subject | Female | en |
dc.subject | Intestine, Small/cytology/drug effects/*metabolism | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | Mitochondria/drug effects/metabolism | en |
dc.subject | Nickel/pharmacology | en |
dc.subject | Patch-Clamp Techniques | en |
dc.subject | Sodium-Calcium Exchanger/antagonists & inhibitors/*metabolism | en |
dc.subject | Thiazepines/pharmacology | en |
dc.title | Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김병주 | - |
dc.contributor.AlternativeAuthor | 전제열 | - |
dc.contributor.AlternativeAuthor | 서인석 | - |
dc.contributor.AlternativeAuthor | 김기환 | - |
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