S-Space College of Medicine/School of Medicine (의과대학/대학원) Microbiology (미생물학전공) Journal Papers (저널논문_미생물학전공)
Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene
- Issue Date
- Microbiol Immunol. 2005;49(6):545-9.
- DNA, Ribosomal/analysis/genetics ; GroEL Protein/*genetics ; Orientia tsutsugamushi/genetics/immunology/*isolation & ; purification/pathogenicity ; Polymerase Chain Reaction ; Scrub Typhus/*epidemiology/immunology ; Typhus, Endemic Flea-Borne/*epidemiology/immunology ; Bacterial Typing Techniques ; Sequence Analysis, DNA
- In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
- 0385-5600 (Print)
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