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Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene

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dc.contributor.authorPark, Hyo-Soon-
dc.contributor.authorLee, Jung-Hee-
dc.contributor.authorJeong, Eun-Ju-
dc.contributor.authorKim, Jung-Eun-
dc.contributor.authorHong, Sung-Jin-
dc.contributor.authorPark, Tae-Kyu-
dc.contributor.authorKim, Tae-Yung-
dc.contributor.authorJang, Won-Jong-
dc.contributor.authorPark, Kyung-Hee-
dc.contributor.authorKim, Bum-Joon-
dc.contributor.authorKook, Yoon-Hoh-
dc.contributor.authorLee, Seung-Hyun-
dc.date.accessioned2010-01-18T05:48:29Z-
dc.date.available2010-01-18T05:48:29Z-
dc.date.issued2005-06-21-
dc.identifier.citationMicrobiol Immunol. 2005;49(6):545-9.en
dc.identifier.issn0385-5600 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15965302-
dc.identifier.urihttps://hdl.handle.net/10371/36676-
dc.description.abstractIn this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subject*Bacterial Typing Techniquesen
dc.subjectDNA, Ribosomal/analysis/geneticsen
dc.subjectGroEL Protein/*geneticsen
dc.subjectOrientia tsutsugamushi/genetics/immunology/*isolation &en
dc.subjectpurification/pathogenicityen
dc.subjectPolymerase Chain Reactionen
dc.subjectScrub Typhus/*epidemiology/immunologyen
dc.subject*Sequence Analysis, DNAen
dc.subjectTyphus, Endemic Flea-Borne/*epidemiology/immunologyen
dc.titleRapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL geneen
dc.typeArticleen
dc.contributor.AlternativeAuthor박효순-
dc.contributor.AlternativeAuthor이정희-
dc.contributor.AlternativeAuthor정은주-
dc.contributor.AlternativeAuthor김정은-
dc.contributor.AlternativeAuthor홍성진-
dc.contributor.AlternativeAuthor박태규-
dc.contributor.AlternativeAuthor장원종-
dc.contributor.AlternativeAuthor박경희-
dc.contributor.AlternativeAuthor김범준-
dc.contributor.AlternativeAuthor국윤호-
dc.contributor.AlternativeAuthor이승현-
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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