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Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Hyo-Soon | - |
dc.contributor.author | Lee, Jung-Hee | - |
dc.contributor.author | Jeong, Eun-Ju | - |
dc.contributor.author | Kim, Jung-Eun | - |
dc.contributor.author | Hong, Sung-Jin | - |
dc.contributor.author | Park, Tae-Kyu | - |
dc.contributor.author | Kim, Tae-Yung | - |
dc.contributor.author | Jang, Won-Jong | - |
dc.contributor.author | Park, Kyung-Hee | - |
dc.contributor.author | Kim, Bum-Joon | - |
dc.contributor.author | Kook, Yoon-Hoh | - |
dc.contributor.author | Lee, Seung-Hyun | - |
dc.date.accessioned | 2010-01-18T05:48:29Z | - |
dc.date.available | 2010-01-18T05:48:29Z | - |
dc.date.issued | 2005-06-21 | - |
dc.identifier.citation | Microbiol Immunol. 2005;49(6):545-9. | en |
dc.identifier.issn | 0385-5600 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15965302 | - |
dc.identifier.uri | https://hdl.handle.net/10371/36676 | - |
dc.description.abstract | In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | DNA, Ribosomal/analysis/genetics | en |
dc.subject | GroEL Protein/*genetics | en |
dc.subject | Orientia tsutsugamushi/genetics/immunology/*isolation & | en |
dc.subject | purification/pathogenicity | en |
dc.subject | Polymerase Chain Reaction | en |
dc.subject | Scrub Typhus/*epidemiology/immunology | en |
dc.subject | Typhus, Endemic Flea-Borne/*epidemiology/immunology | en |
dc.subject | Bacterial Typing Techniques | - |
dc.subject | Sequence Analysis, DNA | - |
dc.title | Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박효순 | - |
dc.contributor.AlternativeAuthor | 이정희 | - |
dc.contributor.AlternativeAuthor | 정은주 | - |
dc.contributor.AlternativeAuthor | 김정은 | - |
dc.contributor.AlternativeAuthor | 홍성진 | - |
dc.contributor.AlternativeAuthor | 박태규 | - |
dc.contributor.AlternativeAuthor | 장원종 | - |
dc.contributor.AlternativeAuthor | 박경희 | - |
dc.contributor.AlternativeAuthor | 김범준 | - |
dc.contributor.AlternativeAuthor | 국윤호 | - |
dc.contributor.AlternativeAuthor | 이승현 | - |
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