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Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene
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- Authors
- Issue Date
- 2005-06-21
- Publisher
- Wiley-Blackwell
- Citation
- Microbiol Immunol. 2005;49(6):545-9.
- Keywords
- DNA, Ribosomal/analysis/genetics ; GroEL Protein/*genetics ; Orientia tsutsugamushi/genetics/immunology/*isolation & ; purification/pathogenicity ; Polymerase Chain Reaction ; Scrub Typhus/*epidemiology/immunology ; Typhus, Endemic Flea-Borne/*epidemiology/immunology ; Bacterial Typing Techniques ; Sequence Analysis, DNA
- Abstract
- In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
- ISSN
- 0385-5600 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15965302
https://hdl.handle.net/10371/36676
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