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Chromosomal constitution of embryos derived from tripronuclear zygotes studied by fluorescence in situ hybridization using probes for chromosomes 4, 13, 18, 21, X, and Y

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dc.contributor.authorPang, Myung-Geol-
dc.contributor.authorJee, Byung-Chul-
dc.contributor.authorKim, Seok-Hyun-
dc.contributor.authorRyu, Buom-Yong-
dc.contributor.authorOh, Sun-Kyung-
dc.contributor.authorSuh, Chang-Suk-
dc.contributor.authorMoon, Shin-Yong-
dc.date.accessioned2010-01-25T12:21:13Z-
dc.date.available2010-01-25T12:21:13Z-
dc.date.issued2005-01-04-
dc.identifier.citationGynecol Obstet Invest. 2005;59(1):14-8. Epub 2004 Aug 18.en
dc.identifier.issn0378-7346 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15627776-
dc.identifier.urihttps://hdl.handle.net/10371/44037-
dc.description.abstractThis study was designed to assess the chromosomal constitution and segregation patterns of cleaving embryos derived from tripronuclear zygotes. Thirty-two embryos obtained from 19 conventional IVF patients were analyzed by fluorescence in situ hybridization (FISH) using probes for chromosomes 4, 13, 18, 21, X, and Y. Sixteen embryos (50.0%) exhibited uniform, non-mosaic patterns. These embryos showed pure triploid (n = 7), pure diploid (n = 7), or pure haploid (n = 2). The remaining 16 embryos showed mosaic patterns; 1 was triploid-diploid mosaics, 9 were diploid-haploid, and 4 were haploid only. Autosomal aneuploidy occurred in 2 embryos showing a triploid complement. The sex chromosomal constituent XXX:XXY:XYY was 3:4:2 in embryos showing a pure triploid complement (including 2 embryos with aneuploidy). This ratio was not significantly different from the expected 1:2:1 (p = 0.96). Pure triploid was found in 41.7% of 2-cell embryos, but no triploid complement was found in 3-cell embryos. The present study also supports the diandric origin of tripronuclear zygotes in the conventional IVF, and, to our knowledge, is the first study to use simultaneous six-color FISH for chromosomes 4, 13, 18, 21, X, and Y in human embryos. However, no additive information was obtained about chromosome 4.en
dc.language.isoen-
dc.publisherKargeren
dc.subject*Chromosome Aberrationsen
dc.subject*Chromosomes, Humanen
dc.subjectEmbryo, Mammalian/*physiologyen
dc.subjectFertilization in Vitroen
dc.subjectHumansen
dc.subjectIn Situ Hybridization, Fluorescence/*methodsen
dc.subject*Sex Chromosomesen
dc.subjectZygoteen
dc.titleChromosomal constitution of embryos derived from tripronuclear zygotes studied by fluorescence in situ hybridization using probes for chromosomes 4, 13, 18, 21, X, and Yen
dc.typeArticleen
dc.contributor.AlternativeAuthor방명걸-
dc.contributor.AlternativeAuthor지병철-
dc.contributor.AlternativeAuthor김석현-
dc.contributor.AlternativeAuthor류범용-
dc.contributor.AlternativeAuthor오선경-
dc.contributor.AlternativeAuthor서창석-
dc.contributor.AlternativeAuthor문신용-
dc.identifier.doi10.1159/000080522-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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