S-Space College of Medicine/School of Medicine (의과대학/대학원) Molecular and Genomic Medicine (분자유전체의학전공) Journal Papers (저널논문_분자유전체의학전공)
Intercellular adhesion molecule-1 is upregulated in ischemic muscle, which mediates trafficking of endothelial progenitor cells
- Yoon, Chang-Hwan; Hur, Jin; Oh, Il-Young; Park, Kyung-Woo; Kim, Tae-Youn; Shin, Jae-Hoon; Kim, Ji-Hyun; Lee, Choon-Soo; Chung, June-Key; Park, Young-Bae; Kim, Hyo-Soo
- Issue Date
- American Heart Association
- Arterioscler Thromb Vasc Biol. 2006 May;26(5):1066-72. Epub 2006 Feb 23.
- Animals; Antigens, CD18/genetics; Antigens, CD31/physiology; Cell Adhesion; Cell Movement; Endothelial Cells/*physiology; Hematopoietic Stem Cells/*physiology; Hindlimb/blood supply; Inflammation/etiology; Intercellular Adhesion Molecule-1/*physiology; Ischemia/*metabolism; Mice; Mice, Inbred C57BL; Muscle, Skeletal/*blood supply; Neovascularization, Physiologic; Stem Cell Transplantation
- BACKGROUND: Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules. METHODS AND RESULTS: Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and platelet-endothelial cell adhesion molecule-1 (PECAM-1) in ischemic muscle by immunofluorescence. ICAM-1 was overexpressed in ischemic muscle compared with nonischemic muscle, whereas expressions of E-selectin, VCAM-1, and PECAM-1 did not show that much difference. ICAM-1 was also upregulated by hypoxia in murine endothelial cells (ECs) as assessed by immunoblot and flow cytometry. EPCs were attached to ECs specifically through ICAM-1/beta-2 integrin interaction in vitro. When EPCs were labeled with fluorescent dye or radioisotope (Tc-99m-HMPAO) and systemically administrated in vivo, EPCs preferentially homed to ischemic muscle. By blocking ICAM-1, EPCs entrapment to ischemic limb in vivo was significantly reduced and neovascularization induced by EPC transplantation was attenuated. CONCLUSIONS: ICAM-1 is upregulated by ischemia, and this is closely associated with EPCs entrapment to ischemic limb. Our findings suggest that ICAM-1 expression might be important in regulating the process of neovascularization through its ability to recruit EPCs.
- 1524-4636 (Electronic)