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Intercellular adhesion molecule-1 is upregulated in ischemic muscle, which mediates trafficking of endothelial progenitor cells
DC Field | Value | Language |
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dc.contributor.author | Yoon, Chang-Hwan | - |
dc.contributor.author | Hur, Jin | - |
dc.contributor.author | Oh, Il-Young | - |
dc.contributor.author | Park, Kyung-Woo | - |
dc.contributor.author | Kim, Tae-Youn | - |
dc.contributor.author | Shin, Jae-Hoon | - |
dc.contributor.author | Kim, Ji-Hyun | - |
dc.contributor.author | Lee, Choon-Soo | - |
dc.contributor.author | Chung, June-Key | - |
dc.contributor.author | Park, Young-Bae | - |
dc.contributor.author | Kim, Hyo-Soo | - |
dc.date.accessioned | 2010-01-25T14:16:06Z | - |
dc.date.available | 2010-01-25T14:16:06Z | - |
dc.date.issued | 2006-02-25 | - |
dc.identifier.citation | Arterioscler Thromb Vasc Biol. 2006 May;26(5):1066-72. Epub 2006 Feb 23. | en |
dc.identifier.issn | 1524-4636 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16497992 | - |
dc.identifier.uri | https://hdl.handle.net/10371/44047 | - |
dc.description.abstract | BACKGROUND: Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules. METHODS AND RESULTS: Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and platelet-endothelial cell adhesion molecule-1 (PECAM-1) in ischemic muscle by immunofluorescence. ICAM-1 was overexpressed in ischemic muscle compared with nonischemic muscle, whereas expressions of E-selectin, VCAM-1, and PECAM-1 did not show that much difference. ICAM-1 was also upregulated by hypoxia in murine endothelial cells (ECs) as assessed by immunoblot and flow cytometry. EPCs were attached to ECs specifically through ICAM-1/beta-2 integrin interaction in vitro. When EPCs were labeled with fluorescent dye or radioisotope (Tc-99m-HMPAO) and systemically administrated in vivo, EPCs preferentially homed to ischemic muscle. By blocking ICAM-1, EPCs entrapment to ischemic limb in vivo was significantly reduced and neovascularization induced by EPC transplantation was attenuated. CONCLUSIONS: ICAM-1 is upregulated by ischemia, and this is closely associated with EPCs entrapment to ischemic limb. Our findings suggest that ICAM-1 expression might be important in regulating the process of neovascularization through its ability to recruit EPCs. | en |
dc.language.iso | en | en |
dc.publisher | American Heart Association | en |
dc.subject | Animals | en |
dc.subject | Antigens, CD18/genetics | en |
dc.subject | Antigens, CD31/physiology | en |
dc.subject | Cell Adhesion | en |
dc.subject | Cell Movement | en |
dc.subject | Endothelial Cells/*physiology | en |
dc.subject | Hematopoietic Stem Cells/*physiology | en |
dc.subject | Hindlimb/blood supply | en |
dc.subject | Inflammation/etiology | en |
dc.subject | Intercellular Adhesion Molecule-1/*physiology | en |
dc.subject | Ischemia/*metabolism | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Muscle, Skeletal/*blood supply | en |
dc.subject | Neovascularization, Physiologic | en |
dc.subject | Stem Cell Transplantation | en |
dc.title | Intercellular adhesion molecule-1 is upregulated in ischemic muscle, which mediates trafficking of endothelial progenitor cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 윤창환 | - |
dc.contributor.AlternativeAuthor | 허진 | - |
dc.contributor.AlternativeAuthor | 오일영 | - |
dc.contributor.AlternativeAuthor | 박경우 | - |
dc.contributor.AlternativeAuthor | 김태윤 | - |
dc.contributor.AlternativeAuthor | 신재훈 | - |
dc.contributor.AlternativeAuthor | 김지현 | - |
dc.contributor.AlternativeAuthor | 이춘수 | - |
dc.contributor.AlternativeAuthor | 정준기 | - |
dc.contributor.AlternativeAuthor | 박영배 | - |
dc.contributor.AlternativeAuthor | 김효수 | - |
dc.identifier.doi | 10.1161/01.ATV.0000215001.92941.6c | - |
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