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Delayed pharmacological pre-conditioning effect of mitochondrial ATP-sensitive potassium channel opener on neurologic injury in a rabbit model of spinal cord ischemia

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dc.contributor.authorKim, Kyoung Ok-
dc.contributor.authorChoe, G-
dc.contributor.authorChung, S H-
dc.contributor.authorKim, C S-
dc.date.accessioned2010-01-27T08:03:56Z-
dc.date.available2010-01-27T08:03:56Z-
dc.date.issued2007-11-17-
dc.identifier.citationActa Anaesthesiol Scand. 2008 Feb;52(2):236-42. Epub 2007 Nov 13.en
dc.identifier.issn1399-6576 (Electronic)-
dc.identifier.issn1399-6576 (Linking)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18005376-
dc.identifier.urihttps://hdl.handle.net/10371/45517-
dc.description.abstractBACKGROUND: Diazoxide, pharmacological openers of mitochondrial ATP-sensitive potassium channels have been shown to induce early pre-conditioning in the spinal cord. Here, the authors investigated whether diazoxide also induce delayed pre-conditioning and thereby reduce neurologic complications using a rabbit model of spinal cord ischemia. METHODS: Infrarenal blood flow was interrupted for 20 min in 21 rabbits. Non-treated control animals received no pre-treatment. Diazoxide (5 mg/kg) were given 48 h before 20 min ischemia in the 48-h DZ group, whereas 15-min DZ group received diazoxide (5 mg/kg) 15 min before 20-min ischemia. Neurological functions were evaluated using Johnson scores for 3 days after reperfusion, after which, spinal cords were procured for hematoxylin and eosin staining for cell counting. RESULTS: Johnson scores revealed a marked improvement in both the diazoxide-treated groups vs. the non-treated control group at 3, 24, 48, and 72 h after reperfusion (P<0.01). The histologic changes were proportional to the Johnson scores, with better preservation of motor neuron numbers in the animals of the 48-h DZ and 15-min DZ group relative to the non-treated controls (81+/-12, 90+/-10, 50+/-23 motor neurons, respectively, P<0.01). No difference was found between the 48-h DZ group and 15-min DZ group with respect to the Johnson scores or neuron numbers. CONCLUSIONS: The study demonstrates that pre-treatment with diazoxide 48 h before ischemia, induce delayed pharmacological pre-conditioning, thereby significantly improving clinical neurologic scores and histologic findings in this animal model.en
dc.language.isoenen
dc.publisherActa Anaesthesiol Scand-
dc.subjectAnalgesics/administration & dosageen
dc.subjectAnimalsen
dc.subjectBlood Gas Analysisen
dc.subjectDiazoxide/*pharmacologyen
dc.subjectDisease Models, Animalen
dc.subjectIschemic Preconditioning/*methodsen
dc.subjectKetamine/administration & dosageen
dc.subjectMaleen
dc.subjectNeurologic Examination/drug effectsen
dc.subjectNeuroprotective Agents/*pharmacologyen
dc.subjectPotassium Channels/*pharmacologyen
dc.subjectRabbitsen
dc.subjectRandom Allocationen
dc.subjectSodium Chloride/administration & dosageen
dc.subjectSpinal Cord/drug effects/physiopathologyen
dc.subjectSpinal Cord Ischemia/*drug therapy/physiopathologyen
dc.subjectTime Factorsen
dc.titleDelayed pharmacological pre-conditioning effect of mitochondrial ATP-sensitive potassium channel opener on neurologic injury in a rabbit model of spinal cord ischemiaen
dc.typeArticleen
dc.contributor.AlternativeAuthor김경옥-
dc.identifier.doi10.1111/j.1399-6576.2007.01534.x-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Anesthesiology and Pain Medicine (마취통증의학전공)Journal Papers (저널논문_마취통증의학전공)
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