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Involvement of glycogen synthase kinase-3beta in palmitate-induced human umbilical vein endothelial cell apoptosis

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dc.contributor.authorChoi, Sung-E-
dc.contributor.authorKang, Yup-
dc.contributor.authorJang, Hyun-Ju-
dc.contributor.authorShin, Ha-Chul-
dc.contributor.authorKim, Hyo-Eun-
dc.contributor.authorKim, Hyo-Soo-
dc.contributor.authorKim, Hae Jin-
dc.contributor.authorKim, Dae Jung-
dc.contributor.authorLee, Kwan-Woo-
dc.date.accessioned2010-01-29T02:04:20Z-
dc.date.available2010-01-29T02:04:20Z-
dc.date.issued2007-05-08-
dc.identifier.citationJ Vasc Res. 2007;44(5):365-74. Epub 2007 May 4.en
dc.identifier.issn1423-0135 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17483602-
dc.identifier.urihttps://hdl.handle.net/10371/46603-
dc.description.abstractBACKGROUND/AIMS: The death of endothelial cells may play a critical role in the development of various vascular diseases, including atherosclerosis. While free fatty acids (FFAs) may stimulate endothelial apoptosis, the molecular and cellular mechanisms of this effect have not been studied intensively. To elucidate the mechanisms involved in FFA-induced endothelial cell apoptosis, we investigated the effect of different pharmacological inhibitors on palmitate-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Interestingly, lithium, a glycogen synthase kinase-3 (GSK-3) inhibitor, showed a strong protective effect. METHODS AND RESULTS: To examine the involvement of GSK-3beta in palmitate-induced HUVEC apoptosis, its dephosphorylation at Ser9 and enzymatic activation in response to palmitate treatment were monitored by immunoblotting and in vitro kinase assays, respectively. GSK-3beta was dephosphorylated and its enzymatic activity increased in palmitate-treated HUVECs. In addition, pretreatment with other GSK-3beta inhibitors, e.g. SB216763 or TDZD-8, as well as adenoviral transduction with a catalytically inactive GSK-3beta had significant protective effects against palmitate-induced HUVEC apoptosis. CONCLUSION: These results demonstrate that the GSK-3beta signalling pathway is involved in palmitate-induced HUVEC apoptosis.en
dc.language.isoenen
dc.publisherKargeren
dc.subjectAdenoviridae/geneticsen
dc.subjectAnthracenes/pharmacologyen
dc.subjectApoptosis/*drug effectsen
dc.subjectCaspase 3/metabolismen
dc.subjectCell Cycle/drug effectsen
dc.subjectCells, Cultured/cytology/drug effectsen
dc.subjectCytosol/chemistryen
dc.subjectEndothelial Cells/cytology/*drug effectsen
dc.subjectEndothelium, Vascular/*cytologyen
dc.subjectEnzyme Activation/drug effectsen
dc.subjectFumonisins/pharmacologyen
dc.subjectGenetic Vectors/pharmacologyen
dc.subjectGlycogen Synthase Kinase 3/antagonists & inhibitors/genetics/*physiologyen
dc.subjectHumansen
dc.subjectImidazoles/pharmacologyen
dc.subjectIndoles/pharmacologyen
dc.subjectLithium Chloride/pharmacologyen
dc.subjectMaleimides/pharmacologyen
dc.subjectMitochondria/chemistryen
dc.subjectPalmitates/*pharmacologyen
dc.subjectPhosphorylationen
dc.subjectPhosphoserine/metabolismen
dc.subjectPoly(ADP-ribose) Polymerases/metabolismen
dc.subjectProtein Processing, Post-Translational/drug effectsen
dc.subjectPyridines/pharmacologyen
dc.subjectThiadiazoles/pharmacologyen
dc.subjectTransduction, Geneticen
dc.subjectUmbilical Veinsen
dc.titleInvolvement of glycogen synthase kinase-3beta in palmitate-induced human umbilical vein endothelial cell apoptosisen
dc.typeArticleen
dc.contributor.AlternativeAuthor최성이-
dc.contributor.AlternativeAuthor강엽-
dc.contributor.AlternativeAuthor장현주-
dc.contributor.AlternativeAuthor심하철-
dc.contributor.AlternativeAuthor김효은-
dc.contributor.AlternativeAuthor김효수-
dc.contributor.AlternativeAuthor김해진-
dc.contributor.AlternativeAuthor김대정-
dc.contributor.AlternativeAuthor이관우-
dc.identifier.doi10.1159/000102321-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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