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Inhibitory effects of 4-n-butylresorcinol on tyrosinase activity and melanin synthesis
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- Authors
- Issue Date
- 2005-12-06
- Publisher
- Pharmaceutical Society of Japan
- Citation
- Biol Pharm Bull. 2005 Dec;28(12):2216-9.
- Keywords
- Animals ; Blotting, Western ; Cell Line ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Down-Regulation/drug effects ; Drug Combinations ; Drug Synergism ; Humans ; Hypopigmentation/chemically induced/metabolism ; Melanins/*antagonists & inhibitors/*biosynthesis/chemistry ; Mice ; Microphthalmia-Associated Transcription Factor/antagonists & ; inhibitors/chemistry/drug effects ; Monophenol Monooxygenase/*antagonists & inhibitors/drug effects/metabolism ; Monoterpenes/chemistry/pharmacology ; Resorcinols/chemistry/*pharmacology ; Signal Transduction/drug effects ; Skin Pigmentation ; Tropolone/analogs & derivatives/chemistry/pharmacology
- Abstract
- In this study, we investigated the effects of 4-n-butylresorcinol on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel-Ab. Our results show that 4-n-butylresorcinol significantly inhibits melanin synthesis in a concentration-dependent manner. In addition, it was also found to inhibit the activity of tyrosinase, the rate-limiting melanogenic enzyme. Several reports have indicated that the activation of extracellular signal-regulated kinase (ERK) or of Akt reduces melanin synthesis via microphthalmia-associated transcription factor (MITF) down-regulation. Accordingly, we examined the effects of 4-n-butylresorcinol on the ERK and Akt signaling pathways. 4-n-Butylresorcinol did not induce ERK, Akt activation, or MITF degradation, and had no effect on cAMP response element binding protein (CREB) phosphorylation, which stimulates MITF expression. In contrast, 4-n-butylresorcinol strongly reduced tyrosinase activity in a cell-free system. Moreover, 4-n-butylresorcinol showed an additive effect in combination with hinokitiol, which reduces MITF expression. These results show that the hypopigmentary effect of 4-n-butylresorcinol results from its direct inhibition of tyrosinase.
- ISSN
- 0918-6158 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16327152
https://hdl.handle.net/10371/46977
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