S-Space College of Medicine/School of Medicine (의과대학/대학원) Molecular and Genomic Medicine (분자유전체의학전공) Journal Papers (저널논문_분자유전체의학전공)
Heat treatment decreases melanin synthesis via protein phosphatase 2A inactivation
- Kim, Dong-Seok; Park, Seo-Hyoung; Kwon, Sun-Bang; Youn, Sang-Woong; Park, Eun-Sang; Park, Kyoung-Chan
- Issue Date
- Cell Signal. 2005 Aug;17(8):1023-31. Epub 2004 Dec 23.
- Animals; Blotting, Western; Butadienes/pharmacology; Cell Line; Cell Survival; DNA-Binding Proteins/metabolism; Enzyme Activation; Enzyme Inhibitors/pharmacology; Extracellular Signal-Regulated MAP Kinases/metabolism; Flavonoids/pharmacology; Hot Temperature; Luciferases/metabolism; Melanins/*biosynthesis; Melanocytes/enzymology/*metabolism; Mice; Microphthalmia-Associated Transcription Factor; Monophenol Monooxygenase/metabolism; Nitriles/pharmacology; Phosphoprotein Phosphatases/*antagonists & inhibitors/*metabolism; Phosphoric Monoester Hydrolases/metabolism; Promoter Regions, Genetic; Protein Phosphatase 2; Temperature; Transcription Factors/metabolism; Transfection
- In the present study, we investigated the effects of heat treatment on melanogenesis in a mouse melanocyte cell line (Mel-Ab). It has been reported that activated extracellular signal-regulated kinase (ERK) is responsible for microphthalmia-associated transcription factor (MITF) degradation, which leads to a reduction in tyrosinase protein production and melanin synthesis. Here we demonstrate that heat treatment induces sustained ERK activation, which may inhibit melanogenesis. However, the specific ERK pathway inhibitors, PD98059 or U0126 did not restore heat-induced hypopigmentation. Furthermore, PD98059 or U0126 hardly blocked the heat-induced activation of ERK. These results suggest that heat treatment may inactivate protein phosphatase, and thus ERK activation is maintained. To support this hypothesis, we examined the effects of heat treatment on protein phosphatase 2A (PP2A) activity. The results obtained show that heat treatment inactivates PP2A, which may subsequently cause ERK activation and that heat treatment inhibits MITF promoter activity. Overall, our results demonstrate that heat treatment reduces melanin production in a temperature-dependent manner.
- 0898-6568 (Print)
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