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Heat treatment decreases melanin synthesis via protein phosphatase 2A inactivation

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Authors
Kim, Dong-Seok; Park, Seo-Hyoung; Kwon, Sun-Bang; Youn, Sang-Woong; Park, Eun-Sang; Park, Kyoung-Chan
Issue Date
2005-05-17
Publisher
Elsevier
Citation
Cell Signal. 2005 Aug;17(8):1023-31. Epub 2004 Dec 23.
Keywords
AnimalsBlotting, WesternButadienes/pharmacologyCell LineCell SurvivalDNA-Binding Proteins/metabolismEnzyme ActivationEnzyme Inhibitors/pharmacologyExtracellular Signal-Regulated MAP Kinases/metabolismFlavonoids/pharmacologyHot TemperatureLuciferases/metabolismMelanins/*biosynthesisMelanocytes/enzymology/*metabolismMiceMicrophthalmia-Associated Transcription FactorMonophenol Monooxygenase/metabolismNitriles/pharmacologyPhosphoprotein Phosphatases/*antagonists & inhibitors/*metabolismPhosphoric Monoester Hydrolases/metabolismPromoter Regions, GeneticProtein Phosphatase 2TemperatureTranscription Factors/metabolismTransfection
Abstract
In the present study, we investigated the effects of heat treatment on melanogenesis in a mouse melanocyte cell line (Mel-Ab). It has been reported that activated extracellular signal-regulated kinase (ERK) is responsible for microphthalmia-associated transcription factor (MITF) degradation, which leads to a reduction in tyrosinase protein production and melanin synthesis. Here we demonstrate that heat treatment induces sustained ERK activation, which may inhibit melanogenesis. However, the specific ERK pathway inhibitors, PD98059 or U0126 did not restore heat-induced hypopigmentation. Furthermore, PD98059 or U0126 hardly blocked the heat-induced activation of ERK. These results suggest that heat treatment may inactivate protein phosphatase, and thus ERK activation is maintained. To support this hypothesis, we examined the effects of heat treatment on protein phosphatase 2A (PP2A) activity. The results obtained show that heat treatment inactivates PP2A, which may subsequently cause ERK activation and that heat treatment inhibits MITF promoter activity. Overall, our results demonstrate that heat treatment reduces melanin production in a temperature-dependent manner.
ISSN
0898-6568 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15894174

http://dx.dor.org/10.1016/j.cellsig.2004.11.020

http://hdl.handle.net/10371/47000
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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