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The signaling network of transforming growth factor β1, protein kinase C delta, and integrin underlies the spreading and invasiveness of gastric carcinoma cells

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dc.contributor.authorLee, Mi-Sook-
dc.contributor.authorKim, Tae Young-
dc.contributor.authorKim, Yong-Bae-
dc.contributor.authorLee, Sung-Yul-
dc.contributor.authorKo, Seong-Gyu-
dc.contributor.authorJong, Hyun-Soon-
dc.contributor.authorKim, Tae-You-
dc.contributor.authorBang, Yung-Jue-
dc.contributor.authorLee, Jung Weon-
dc.date.accessioned2010-01-29T15:19:46Z-
dc.date.available2010-01-29T15:19:46Z-
dc.date.issued2005-08-
dc.identifier.citationMolecular and Cellular Biology, Vol.25 No.16, pp.6921-6936-
dc.identifier.issn0270-7306-
dc.identifier.other3925-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16055706-
dc.identifier.urihttp://hdl.handle.net/10371/47146-
dc.description.abstractIntegrin-mediated cell adhesion and spreading enables cells to respond to extracellular stimuli for cellular functions. Using a gastric carcinoma cell line that is usually round in adhesion, we explored the mechanisms underlying the cell spreading process, separate from adhesion, and the biological consequences of the process. The cells exhibited spreading behavior through the collaboration of integrin-extracellular matrix interaction with a Smad-mediated transforming growth factor PI (TGF beta 1) pathway that is mediated by protein kinase C delta (PKC delta). TGF beta 1 treatment of the cells replated on extracellular matrix caused the expression and phosphorylation of PKC delta, which is required for expression and activation of integrins. Increased expression of integrins alpha 2 and alpha 3 correlated with the spreading, functioning in activation of focal adhesion molecules. Smad3, but not Smad2, overexpression enhanced the TGF beta 1 effects. Furthermore, TGF beta 1 treatment and PKC delta activity were required for increased motility on fibronectin and invasion through matrigel, indicating their correlation with the spreading behavior. Altogether, this study clearly evidenced that the signaling network, involving the Smad-dependent TGF beta pathway, PKC delta expression and phosphorylation, and integrin expression and activation, regulates cell spreading, motility, and invasion of the SNU16mAd gastric carcinoma cell variant.-
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.subjectBlotting, Westernen
dc.subjectCell Adhesionen
dc.subjectCell Lineen
dc.subjectCell Line, Tumoren
dc.subjectCell Movementen
dc.subjectCollagen/pharmacologyen
dc.subjectDrug Combinationsen
dc.subjectExtracellular Matrix/metabolismen
dc.subjectFibronectins/metabolismen
dc.subjectFlow Cytometryen
dc.subjectFluorescent Antibody Technique, Indirecten
dc.subjectGreen Fluorescent Proteins/metabolismen
dc.subjectHumansen
dc.subjectImmunoprecipitationen
dc.subjectIntegrin alpha2/metabolismen
dc.subjectIntegrin alpha3/metabolismen
dc.subjectLaminin/pharmacologyen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectModels, Biologicalen
dc.subjectNeoplasm Invasivenessen
dc.subjectPhosphorylationen
dc.subjectProtein Kinase C/*metabolismen
dc.subjectProtein Kinase C-deltaen
dc.subjectProteoglycans/pharmacologyen
dc.subjectRNA, Small Interfering/metabolismen
dc.subject*Signal Transductionen
dc.subjectStomach Neoplasms/metabolism/*pathologyen
dc.subjectTime Factorsen
dc.subjectTransfectionen
dc.subjectTransforming Growth Factor beta/*metabolismen
dc.subjectTransforming Growth Factor beta1en
dc.subjectWound Healingen
dc.titleThe signaling network of transforming growth factor β1, protein kinase C delta, and integrin underlies the spreading and invasiveness of gastric carcinoma cells-
dc.typeArticle-
dc.contributor.AlternativeAuthor방영주-
dc.identifier.doi10.1128/MCB.25.16.6921-6936.2005-
dc.citation.journaltitleMolecular and Cellular Biology-
dc.identifier.scopusid2-s2.0-23344448600-
dc.citation.endpage6936-
dc.citation.number16-
dc.citation.startpage6921-
dc.citation.volume25-
dc.identifier.urlhttps://mcb.asm.org/content/25/16/6921-
dc.identifier.rimsid3925-
dc.identifier.sci000231000800007-
dc.contributor.affiliatedAuthorBang, Yung-Jue-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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