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Elucidation of CPX-1 involvement in RANKL-induced osteoclastogenesis by a proteomics approach

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dc.contributor.authorPark, Joo-Cheol-
dc.contributor.authorChang, Eun-Ju-
dc.contributor.authorKwak, Han-Bok-
dc.contributor.authorKim, Hyunsoo-
dc.contributor.authorLee, Zang-Hee-
dc.contributor.authorKim, Hong-Hee-
dc.date.accessioned2010-02-01T06:03:03Z-
dc.date.available2010-02-01T06:03:03Z-
dc.date.issued2004-04-
dc.identifier.citationFEBS LETTERS. 564, 166-170.en
dc.identifier.issn0014-5793-
dc.identifier.urihttps://hdl.handle.net/10371/47255-
dc.description.abstractTo identify proteins potentially involved in osteoclast differentiation, we conducted a proteomics-based analysis using the osteoclastogenesis model cell line RAW264.7. Total proteins from undifferentiated cells, committed pre-osteoclasts, and differentiated osteoclasts were resolved by two-dimensional gel electrophoresis. Protein spots showing differential expression levels were processed for peptide mass fingerprinting. Among them, we identified the metallocarboxypeptidase CPX-1, which was prominently increased in pre-osteoclasts and then decreased in mature osteoclasts. Results of reverse transcription polymerase chain reaction, Western blot, and confocal microscopy were in agreement with the proteomics data. Notably, the forced overexpression of CPX-1 led to the inhibition of osteoclast formation, but not pre-osteoclast generation. Therefore, the transient up-regulation pattern of CPX-1 expression may be important for the successful progression from pre-osteoclasts to mature osteoclasts.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectosteoclasten
dc.subjectranklen
dc.subjectcpx-1en
dc.subjectproteomicsen
dc.titleElucidation of CPX-1 involvement in RANKL-induced osteoclastogenesis by a proteomics approachen
dc.typeArticleen
dc.contributor.AlternativeAuthor박주철-
dc.contributor.AlternativeAuthor장은주-
dc.contributor.AlternativeAuthor곽한복-
dc.contributor.AlternativeAuthor김현수-
dc.contributor.AlternativeAuthor이장희-
dc.contributor.AlternativeAuthor김홍희-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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