5-Lipoxygenase 대사산물들이 파골세포 생성에 미치는 영향

Abstract

Since 5-iipoxygenase metabolites production is known, to be enhanced in inflammed and malignant tissues which are associated with local bone destruction, ´ it is likely that 5iipoxygenase metabolites play some ro´,es in bone destruction. Therefore .ve performed this study to ´find out the effect of 5lipoxygenase metabolites on the osteoclast formation. Coculture of neonatal mouse calvarial osteoblastic cells and mouse bone ma-row cells was established and cultured for 7 days. After the culture, staining for tartrateresistant ac´ol phosphatase, a marker enzyme of osteoclast, was executed and the number of tartrateresistant acid ´phosphatase positive multinucleated cells was counted. 1,25-dhydroxyvitamn D; and tumor necrosis factor a significantly stimulated the osteoclast formation. When 5lipoxygenase pathway was blocked by caffeic acid, 1,25dihydroxyvitamin D, or tumor ´necrosis factor- a -induced osteoclast formation was significantly inhibited. Furthermore, when both cyclooxygenase and 54poxygenase pathway were blocked by combined treatment of indomethaoin and caffeic acid, 1,25dhydroxyvitamin D, o-tumor necrosis factor a -induced osteoclast formation was almost completely inhibited. Though phospholipase A, or meilitin failed to induce osteoclast formation- sy themselves, they significantly enhanced 1,25dihydroxyvitamin D:induced osteoclast formation. And caffeic acid partly , inh!Lted the osteoclast formation induced by comD´ned t7eatment of phospholipase A, and 125dihydroxyvitarrin D, or meliftur and 1,25dihydroxyvitamin D;. Taken toge her, 5lipoxygenase metabolites seem to have certain role(3) in the osteoc:ast formation induced by 1,25dihydroxyvitamin D; or tumor necrosis factora and in the local bone destruction associated with inflammation by enhancing osteoclast fDrmation.