의치상 레진의 세포독성에 관한 연구

Abstract

The purpose of this study was to investigate the cytotoxicity and mutagenicity of denture base resins. According to manufacturer's instructions, resin specimens were made. Group 1 : heat-polymerizing acrylic resin (Luciton ) Group 2 : heat-polymerizing acrylic resin containing polyhedraloligosilsesquioxane(POSS resin) Group 3 : auto-polymerizing acrylic resin (Repair ) Group 4 : direct relining auto-polymerizing acrylic resin (Tokuso ). Fresh specimens 24 hrs. and 72 hrs. soaked specimens in distil)ed water were made. Responses with metabolic assay and mutagenesis assay to eluates from resin specimens were measured. Cultures with medium alone provided controls. Cytotoxicity was assessed with agar overlay test. The results were as follows; 1. Group 4 showed higher cytotoxicity than Group 1, Group 2 and Group 3 in fresh, 24-an4 72-hour immersion caries (p<.05). Group 3 showed higher cytotoxicity than Group 2 in fresh cases and showed higher cytotoxicity than Group 1 and Group 2 in 24-and 72-hour immersion cases (p<.05) . Group 1 and Group 2 showed no significant difference. 2. All acrylic denture base resins skewed significant increase of cell activity as immersion time increased (p<.05). 3. Auto-polymerizing acrylic denture base resins skewed higher cytotoxicity than heat-polymerizing acrylic denture base resins (p<.05). 4. All acrylic denture base resins showed lower mutagenicity than controls (p<.05).