쥐의 포르말린시험에서 척수에서의 열충격 단백질들의 발현에 관한 연구

Abstract

Background: Heat shock proteins (HSPs) are induced in the central nervous system by stressful stimuli are thought to assist in the maintenance of cellular integrity and viability. Recently, several lines of evidence have indicated that HSPs are related to the synaptic in the nervous system, which is related to the nociceptive stimuli induced by formalin injection into rat's hind paw. Methods: Male Sprague-Dawley rats weighing 250-300g were subcutaneously injected with 100㎕ of fershly made 5% formalin into the right hind paw. At 1 and 24 hour(s) after formalin injection, the right and left sides of the 5^th and 6^th lumbar spinal cord were collected. HSP90, HSP 70, and HSP27 levels were examined using Western blot analysis. Results: Pain related behaviors induced by formalin were typically observed during 1 hr after formalin injection. However, at 24 hr after formalin injeciton, such pain behaviors were rarely observed. Only at 1 hr after formalin injection, HSP70 was significantly more expressed in the ipsilateral and contralateral lumbar spinal cord (P ＜0.05). The others did not show significant changes in HSPs levels compared to those of the control group. Conclusions: These results suggest that the overexpression of HSP70 in the lumbar spinal cord at 1 hr after formalin injection, which is associated with painful stress, may be related to the molecular mechanism of central sensitization.