S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 27 No.1 (1986)
Production of Monoclonal Antibody to Oxytocin for Immunohistochemical Use
Oxytocin에 대한 단세포군 항체 생산 및 면역조직화학적 이용에 관한 연구
- Lee, Seung Ro; Cho, Sa Sun
- Issue Date
- Seoul J Med, Vol.27 No.1, pp. 19-26
- This study was performed to develop a hybrid cell line secreting antibody to
oxytocin for immunohistochemical localization of oxytocin neuron in the brain.
Synthetic oxytocin was conjugated to bovine thyroglobulin and used in immunization of
Balb/c mice. The spleen cells from immunized mice were fused with NS1 murine myeloma
cells using polyethylene glycol, and the hybrid cells were selected by HAT medium.
A radiobinding test was used to detect the presence of antibody in the culture fluid of
hybrids, and specificity of the antibodies was characterized by both modified enzyme linked
immunosorbent assay and immunocytochemical method.
Two hybrid cell lines were found to secrete specific anti-oxytocin antibodies.
These monospecific antibodies did not cross-react with arginine vasopressin or vasotocin.
By light immunocytochemistry these antibodies were able to localize differentially the oxytocin
neurons from the vasopressin neurons in the paraventricular nucleus, supraoptic nucleus,
median eminence and posterior pituitary gland.