Association of Oncogenic Human Papillomaviruses(HPV 16, 18) with Cervical Intraepithelial Neoplasia and Cervical Cancer

Abstract

The prevalence of oncogenic human papillomavirus (HPV) type 16 and 18 was investigated by polymerase chain reaction (PCR) method in cervical scrapes omitting prior DNA extraction. Samples were obtained from 70 gynecologic inpatients with normal cervix and 160 women with cervical neoplastic lesion ( N = 50 in cervical intraepithelial neoplasia (CIN) I, N = 50 in CIN II, N = 30 in CIN III, N = 30 in invasive cervical cancer). Eight members were excluded from the data due to failure of ,f3-g10bin amplification during the PCR procedure. The HPV 16 prevalence rate was 19.1 % (13/68) in the normal group, 38.8 % (19/49) in CIN 1,57.1 % (28/49) in CIN 11,75.9 % (22/29) in CIN III, 88.9% (24/27) in invasive cancer. For HPV type 18, DNA positivity was 4.4 % (3/68), 8.2 % (4/49), 12.2 % (6/49), 13.8 % (4/29), 18.5 % (5/27), respectively. In the whole series a consistent correlation was found between HPV positivity and severity of cervical lesion. HPV 16 was the more prevalent type and about five times more common than HPV 18. These results suggest that HPV 16 and 18 may be strongly associated with carcinogenesis of cervical cancer. The high risk HPV typing by direct PCR from cervical scrapes can be used as a useful marker for the presence of neoplastic cells and also served as a simple tool in identifying women who are at risk of developing dysplasia and cervical cancer.