Studies on a Putative (Na+, K+)-ATPase Inhibitor in Chronic Renal Failure Patient's Serum : 만성 신부전증 환자혈액내의 (Na+, K+)-ATPase 억제물질에 관한 연구

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Lee, Hong Kyu; Park, Young-Sik; Kim, Seong Yeon; Lee, Jung Sang

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Seoul National University College of Medicine
Seoul J Med, Vol.26 No.1, pp. 64-68
Circulating inhibitor(Na+, K+ )-ATPaseChronic renal failureRat kidney membraneNaturetic hormoneHypertension
In order to identify and purify a putative substance which might inhibit (Na +, K
+)-ATPase in vitro assay sytem, serum extracts from normal persons and C.RF.(chronic renal
failure) patients were chromatographed and active peaks were compared with each other. Rat
kidney membrane was partially purified with a sucrose step density gradient ultracentrifugation
and used for (Na +, K+)-ATPase source. Deproteinized serum obtained by acidification and
boiling was gel chromatographed on Sephadex G-25 column in order to remove salts and
inorganic phosphate in the sample which interfered with the assay system. Two active peaks
appeared just prior to the total volume and in the total volume fractions, and the former was
further investigated.
Collected fractions were concentrated with freeze-dryer and rechromatorgraphed on
Sephadex G-75-120. One major active peak at total volume fractions was separated to
several active peaks by using cation exchange CM-Sephadex C-25 column. These peaks from
normal and C.RF. serum were compared with each other respectively. The eluted patterns
were similar to each other except that one peak appeared at three fifths of total elution
volume was shown to be more active in C.RF. serum than that in normal serum.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 26 No.1 (1985)
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