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Capsaicin inhibits phospholipase C-mediated Ca(2+) increase by blocking thapsigargin-sensitive store-operated Ca(2+) entry in PC12 cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2010-02-25T04:08:21Z | - |
dc.date.available | 2010-02-25T04:08:21Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Pharmacology and Experimental Therapeutics 291:107–114 | en |
dc.identifier.issn | 0022-3565 | - |
dc.identifier.uri | https://hdl.handle.net/10371/58452 | - |
dc.description.abstract | Capsaicin has been shown to act through vanilloid receptors,
which are temperature-sensitive cation channels. However, there also are indications that suggest the capsaicin effect is not mediated by the vanilloid receptor. We therefore investigated the effect of capsaicin on the phospholipase C-mediated Ca21 rise in PC12 cells. Capsaicin caused a rapid decline in extracellular ATP- or bradykinin-induced calcium transients to the basal level without significant attenuation of the peak level. However, capsaicin did not inhibit either ATP- or bradykinininduced Ca21 elevation in the absence of extracellular Ca21 or inositol-1,4,5-trisphosphate production. Capsaicin also inhibited ATP-induced norepinephrine secretion. Capsaicin dramatically reduced the thapsigargin-induced sustained Ca21 level, suggesting that capsaicin inhibits thapsigargin-sensitive storeoperated Ca21 entry (SOCE). Thapsigargin-induced Ba21 and Mn21 influx was also inhibited by capsaicin. Furthermore, capsaicin overlapped SK&F96365 in inhibiting thapsigargin-sensitive SOCE. Capsaicin-induced inhibition of SOCE also occurred in thapsigargin-treated Jurkat-T cells, which have a rather prominent SOCE. Resiniferatoxin, a vanilloid receptor agonist, did not mimic the effect of capsaicin. Ruthenium red and capsazepine, which are known to inhibit the vanilloid receptor, did not affect this capsaicin effect. The results suggest that capsaicin does not mediate vanilloid receptor signaling when inhibiting the thapsigargin-sensitive SOCE. The capsaicin action was also not mediated by activation of protein kinase C because phorbol-12-myristate 13-acetate and capsaicin did not overlap each others effect and GF109203X did not reverse the inhibitory effect of capsaicin. The results suggest that capsaicin negatively modulates thapsigargin-sensitive SOCE subsequent to phospholipase C activation. | en |
dc.description.sponsorship | This work was supported by the Korea Research Foundation and the
Ministry of Science and Technology (98-J04-02-05-A-06). We are also grateful for the support from the Brain Research Program of the Ministry of Science and Technology. | en |
dc.language.iso | en | - |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics (ASPET) | en |
dc.title | Capsaicin inhibits phospholipase C-mediated Ca(2+) increase by blocking thapsigargin-sensitive store-operated Ca(2+) entry in PC12 cells. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
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