Protein Immobilization on Aminated Poly(glycidyl methacrylate) Nanofibers as Polymeric Carriers

Abstract

Recently, protein carriers based on nanomaterials have been highlighted in diverse biological applications such as protein extraction, separation, and delivery due to their facile gravimetric sedimentation in the aqueous phase and abundant surface functionalities, which were used as anchoring sites for proteins. From this viewpoint, poly- (glycidyl methacrylate) nanofibers (PGMA NFs) can be an excellent candidate for protein support because PGMA NFs possess the activated epoxide functional groups on the surface. In addition, cured PGMA NFs (PGMA-NH2 NFs) reveal different surface functionalities such as primary amine groups. They can be linked with carboxylated proteins. Ferritin and streptavidin were selected as models of the pristine and biolinker-mediated proteins in this experiment and immobilized onto PGMA NFs and aminated PGMA-NH2 NFs. The successful conjugations of ferritin and streptavidin were confirmed with transmission electron microscopy and fluorescein-isothiocyanatetagged molecules. Protein immobilization using the pristine and the cured PGMA NFs could be considered as an outstanding protocol for facile protein delivery.