Creation and Viability of an Isolated Jejunal Segment Using the Omentum as Vascular Pedlcle

Abstract

To evaluate the feasibility of the creation of an Isolated Jejunal Segment (IJS) which is devoided of its anatomical blood supply and nourished by the omentum, the experimental loop was created in rats and its viability was examined microscopically. The procedure of creating IJS is: Llliqation of the mesenteric vessels and the arcade vessels of a jejunal segment, 2)fixation of the omentum to the serosa of the segmentiabout 3 em ), 3) 6 weeks after, isolation of the segment by division of both ends. At the same time bowel continuity was restored, and the IJS was left in the peritoneal cavity freely. For evaluation of viability the experimental segment was harvested 2 weeks after creation and examined microscopically after hematoxylin and eosin staining. All 13 IJSs were viable, 10 showed normal architecture and 3 showed atrophy of the mucosa and muscle layer and dilatation of the lumen filled with mucus . In conclusion an isolated bowel segment can be created using omentopexy technique.