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Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult

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dc.contributor.authorHwang, In Koo-
dc.contributor.authorYoo, Ki-Yeon-
dc.contributor.authorNam, Young Sam-
dc.contributor.authorChoi, Jung Hoon-
dc.contributor.authorLee, In Se-
dc.contributor.authorKwon, Young-Guen-
dc.contributor.authorKang, Tae-Cheon-
dc.contributor.authorKim, Yong-Sun-
dc.contributor.authorWon, Moo Ho-
dc.date.accessioned2009-08-07T02:50:45Z-
dc.date.available2009-08-07T02:50:45Z-
dc.date.issued2006-02-13-
dc.identifier.citationNeurosci. Res. 54, 319-327en
dc.identifier.issn0168-0102-
dc.identifier.urihttps://hdl.handle.net/10371/6477-
dc.description.abstractIn the present study, we observed expression and changes of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the gerbil hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia. In blood, corticosterone levels were increased biphasically at 30 min and 12 h after ischemia/reperfusion, and thereafter its levels were decreased. In the sham-operated group, MR and GR immunoreactivities were weakly detected in the CA1 region. By 3 days after ischemia, MR and GR were not significantly altered in the CA1 region: at 12 h after ischemia, GR was expressed in a few neurons in the CA1 region, whereas MR was not expressed in any neurons after ischemic insult. From 4 days after ischemia, MR and GR immunoreactivities were detected in astrocytes and microglia in the CA1 region, and at 7 days after ischemia, MR and GR immunoreactivities peaked in the hippocampal CA1 region. At this time, 55% of astrocytes and 30% of microglia showed MR immunoreactivity, and 20% of astrocytes and 40% of microglia showed GR immunoreactivity. Western blot analyses showed that the pattern of changes in MR and GR protein levels was similar to the immunohistochemical changes observed after transient forebrain ischemia. From 4 days after ischemia, MR and GR protein levels were increased time-dependently after ischemia. In conclusion, enhanced MR and GR expressions in astrocytes and microglia were detected in the hippocampal CA1 region 4–7 days after ischemia/reperfusion. At this time, GR immunoreactivity was abundant in microglia, whereas MR immunoreactivity was prominent in astrocytes. The specific distribution of corticosteroid receptors in the astrocytes and microglia may be associated with the differences of MR and GR functions against ischemic damage.en
dc.description.sponsorshipThe authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help for this study. This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A020007).en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectTransient forebrain ischemiaen
dc.subjectHippocampal CA1 regionen
dc.subjectMineralocorticoid receptoren
dc.subjectGlucocorticoid receptoren
dc.subjectGliosisen
dc.subjectAstrocytesen
dc.subjectMicrogliaen
dc.subjectGerbilen
dc.titleMineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insulten
dc.typeArticleen
dc.contributor.AlternativeAuthor황인구-
dc.contributor.AlternativeAuthor유기연-
dc.contributor.AlternativeAuthor남영삼-
dc.contributor.AlternativeAuthor최정훈-
dc.contributor.AlternativeAuthor이인세-
dc.contributor.AlternativeAuthor권영근-
dc.contributor.AlternativeAuthor강태천-
dc.contributor.AlternativeAuthor김용선-
dc.contributor.AlternativeAuthor원무호-
dc.identifier.doi10.1016/j.neures.2005.12.012-
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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