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EGF-induced inhibition of glucose transport is mediated by PKC and MAPK signal pathways in primary cultured chicken hepatocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Min Young | - |
dc.contributor.author | Park, Soo Hyun | - |
dc.contributor.author | Lee, Yun Jung | - |
dc.contributor.author | Heo, Jung Sun | - |
dc.contributor.author | Lee, Jang-Hern | - |
dc.contributor.author | Han, Ho Jae | - |
dc.date.accessioned | 2009-08-07T04:11:22Z | - |
dc.date.available | 2009-08-07T04:11:22Z | - |
dc.date.issued | 2006-06-15 | - |
dc.identifier.citation | Am J Physiol Gastrointest Liver Physiol 291: G744–G750, 2006 | en |
dc.identifier.issn | 0193-1857 (print) | - |
dc.identifier.issn | 1522-1547 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/6502 | - |
dc.description.abstract | EGF is a regulator of a wide variety of processes in various cell systems. Hepatocytes are important sites in the body's metabolism and function. Glucose transporter 2 (GLUT2) is a major transporter that is expressed strongly in hepatocytes. Therefore, this study examined the effect of EGF on GLUT2 and its related signal cascades in primary cultured chicken hepatocytes. EGF decreased [3H]deoxyglucose uptake in a dose- and time-dependent manner (>10 ng/ml, 2 h). AG-1478 (an EGF receptor antagonist) and genistein and herbimycin A (tyrosine kinase inhibitors) blocked the EGF-induced decrease in [3H]deoxyglucose uptake, which correlated with the GLUT2 expression level. In addition, the EGF-induced decrease in GLUT2 protein expression was inhibited by staurosporine, H-7, or bisindolylmaleimide I (PKC inhibitors), PD-98059 (a MEK inhibitor), SB-203580 (a p38 MAPK inhibitor), and SP-600125 (a JNK inhibitor), suggesting a role of both PKC and MAPKs (p44/42 MAPK, p38 MAPK, and JNK). In particular, EGF increased the translocation of PKC isoforms (PKC-, -1, -, -, and -) from the cytosol to the membrane fraction and increased the activation of p44/42 MAPK, p38 MAPK, and JNK. Moreover, PKC inhibitors blocked the EGF-induced phosphorylation of three MAPKs. In conclusion, EGF decreases the GLUT2 expression level via the PKC-MAPK signal cascade in chicken hepatocytes. | en |
dc.description.sponsorship | This research was supported by the Korean Rural Development Administration
(BioGreen 21 Program), and the authors acknowledge a graduate fellowship provided by the Ministry of Education and Human Resources Development through the Brain Korea 21 project, Republic of Korea. | en |
dc.language.iso | en | - |
dc.publisher | American Physiological Society | en |
dc.subject | epidermal growth factor | en |
dc.subject | glucose transporter 2 | en |
dc.subject | protein kinase C | en |
dc.subject | mitogen-activated protein kinases | en |
dc.title | EGF-induced inhibition of glucose transport is mediated by PKC and MAPK signal pathways in primary cultured chicken hepatocytes | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이민영 | - |
dc.contributor.AlternativeAuthor | 박수현 | - |
dc.contributor.AlternativeAuthor | 이윤정 | - |
dc.contributor.AlternativeAuthor | 허정선 | - |
dc.contributor.AlternativeAuthor | 이장헌 | - |
dc.contributor.AlternativeAuthor | 한호재 | - |
dc.identifier.doi | 10.1152/ajpgi.00551.2005 | - |
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