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In vivo bioluminescence imaging of cord blood derived mesenchymal stem cell transplantation into rat myocardium
DC Field | Value | Language |
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dc.contributor.author | Min, Jung-Joon | - |
dc.contributor.author | Ahn, Youngkeun | - |
dc.contributor.author | Moon, Sungmin | - |
dc.contributor.author | Kim, Yong Sook | - |
dc.contributor.author | Park, Jong Eun | - |
dc.contributor.author | Kim, Sung Mi | - |
dc.contributor.author | Le, Uyenchi N. | - |
dc.contributor.author | Wu, Joseph C. | - |
dc.contributor.author | Joo, Soo Yeon | - |
dc.contributor.author | Hong, Moon Hwa | - |
dc.contributor.author | Yang, Deok Hwan | - |
dc.contributor.author | Jeong, Myung Ho | - |
dc.contributor.author | Song, Chang Hun | - |
dc.contributor.author | Jeong, Yun Hyeok | - |
dc.contributor.author | Yoo, Kyung Yeon | - |
dc.contributor.author | Kang, Kyung-Sun | - |
dc.contributor.author | Bom, Hee-Seung | - |
dc.date.accessioned | 2009-08-07T06:52:45Z | - |
dc.date.available | 2009-08-07T06:52:45Z | - |
dc.date.issued | 2006-04 | - |
dc.identifier.citation | Ann Nucl Med. 2006; 20: 165-170 | en |
dc.identifier.issn | 0914-7187 (print) | - |
dc.identifier.issn | 1864-6433 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/6521 | - |
dc.description.abstract | The conventional method for the analysis of myocardial cell transplantation depends on
postmortem histology. Here, we have sought to demonstrate the feasibility of a longitudinal monitoring of transplanted cell survival in living animals, accomplished with optical imaging techniques and pharmacological interventions. Methods: Human cord blood (50 ml) was donated with parental consent. After getting cord blood derived mesenchymal stem cells (CBMSCs), cells were transfected (MOI = 100) overnight with adenovirus encoding firefly luciferase gene (Ad- CMV-Fluc). Our experimental Sprague-Dawley rats (n = 12) were given intramyocardial injections containing 1 × 106 CBMSCs, which had been made to express the firefly luciferase (Fluc) reporter gene. Optical bioluminescence imaging was then conducted using a cooled charged-coupled device (CCD) camera (Xenogen), beginning on the day after the transplantation (day 1). Groups of mice were intraperitoneally injected with cyclosporine (5 mg/kg) or tacrolimus (1 mg/kg), in an attempt to determine the degree to which cell survival had been prolonged, and these values were then compared with the cell survival values of the negative control group. The presence of transplanted CBMSCs on in vivo images confirmed by in situ hybridization for human specific Alu in the myocardium. Results: Cardiac bioluminescence signals were determined to be present for 6 days after transplantation: day 1 (97000 ± 9100 × 105 p/s/cm2/sr), day 3 (9600 ± 1110 p/s/cm2/sr), and day 5 (3200 ± 550 p/s/cm2/sr). The six mice that received either cyclosporine or tacrolimus displayed cardiac bioluminescence signals for a period of 8 days after transplantation. We observed significant differences between the treated group and the non-treated group, beginning on day 3 (tacrolimus; 26500 ± 4340 p/s/cm2/sr, cyclosporine; 27200 ± 3340 p/s/cm2/sr, non-treated; 9630 ± 1180 p/s/cm2/sr, p < 0.01), and persisting until day 7 (tacrolimus; 12500 ± 2946 p/s/cm2/sr, cyclosporine; 7310 ± 1258 p/s/cm2/sr, non-treated; 2460 ± 160 p/s/cm2/sr, p < 0.01). The humanderived CBMSCs were detected in the myocardium 3 days after transplantation by in situ hybridization. Conclusions: The locations, magnitude, and survival duration of the CBMSCs were noninvasively monitored with a bioluminescence optical imaging system. We determined that optical molecular imaging expedites the fast throughput screening of pharmaceutical agents, allowing for the noninvasive tracking of cell survival within animals. In rat cardiac CBMSC transplant models, transient immunosuppressive treatment with tacrolimus or cyclosporine was shown to improve donor cell survival. | en |
dc.description.sponsorship | This work was supported by a grant from the Nuclear Energy
R&D program (M20203200028-02A0702-00411) from the Ministry of Science and Technology of Korea. This study was financially supported by Chonnam National University in 2005. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | cord blood | en |
dc.subject | mesenchymal stem cell | en |
dc.subject | bioluminescence | en |
dc.subject | molecular imaging | en |
dc.title | In vivo bioluminescence imaging of cord blood derived mesenchymal stem cell transplantation into rat myocardium | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 민정준 | - |
dc.contributor.AlternativeAuthor | 안영근 | - |
dc.contributor.AlternativeAuthor | 문성민 | - |
dc.contributor.AlternativeAuthor | 김용숙 | - |
dc.contributor.AlternativeAuthor | 박종은 | - |
dc.contributor.AlternativeAuthor | 김성미 | - |
dc.contributor.AlternativeAuthor | 주수연 | - |
dc.contributor.AlternativeAuthor | 홍문화 | - |
dc.contributor.AlternativeAuthor | 양덕환 | - |
dc.contributor.AlternativeAuthor | 정명호 | - |
dc.contributor.AlternativeAuthor | 송창훈 | - |
dc.contributor.AlternativeAuthor | 정윤혁 | - |
dc.contributor.AlternativeAuthor | 유경연 | - |
dc.contributor.AlternativeAuthor | 강경선 | - |
dc.contributor.AlternativeAuthor | 범희승 | - |
dc.identifier.doi | 10.1007/BF03027425 | - |
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