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One-Step Immunochromatography Assay Kit for Detecting Antibodies to Canine Parvovirus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Jin-Sik | - |
dc.contributor.author | Ha, Gun-Woo | - |
dc.contributor.author | Cho, Young-Shik | - |
dc.contributor.author | Kim, Min-Jae | - |
dc.contributor.author | An, Dong-Jun | - |
dc.contributor.author | Hwang, Kyu-Kye | - |
dc.contributor.author | Lim, Yoon-Kyu | - |
dc.contributor.author | Park, BongKyun | - |
dc.contributor.author | Kang, BoKyu | - |
dc.contributor.author | Song, Dae-Sub | - |
dc.date.accessioned | 2009-08-07T08:13:05Z | - |
dc.date.available | 2009-08-07T08:13:05Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Clin. Vaccine Immunol. 13:520-524 | en |
dc.identifier.issn | 1071-412X | - |
dc.identifier.uri | https://hdl.handle.net/10371/6546 | - |
dc.description.abstract | This study was performed to determine the feasibility of using whole serum to detect antibodies to canine
parvovirus (CPV) under nonlaboratory conditions and to evaluate the performance characteristics of an immunochromatography assay kit. Precise detection of levels of antibody against CPV in puppies can be used to determine a vaccination schedule, because maternal antibodies frequently result in the failure of protective vaccination, and can also be used to determine the antibody levels of infected puppies. Several methods for the titration of CPV antibodies have been reported, including the hemagglutination inhibition (HI) assay, which is considered the gold standard. These methods, however, require intricate and time-consuming procedures. In this study, a total of 386 serum specimens were tested. Compared to the HI assay, the rapid assay had a 97.1% sensitivity and a 76.6% specificity (with a cutoff HI titer of 1:80). This single-step assay could be performed rapidly and easily without special equipment. The kit provides a reliable method for detection of anti-CPV antibody where laboratory support and personnel are limited. | en |
dc.description.sponsorship | We are very grateful for the highly skilled and enthusiastic support
of J. E. Yoo and K. S. Na. Their input and dedication helped to make this work possible. | en |
dc.language.iso | en | - |
dc.publisher | American Society for Microbiology | en |
dc.title | One-Step Immunochromatography Assay Kit for Detecting Antibodies to Canine Parvovirus | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 오진식 | - |
dc.contributor.AlternativeAuthor | 하건우 | - |
dc.contributor.AlternativeAuthor | 조영식 | - |
dc.contributor.AlternativeAuthor | 김민재 | - |
dc.contributor.AlternativeAuthor | 안동준 | - |
dc.contributor.AlternativeAuthor | 황규계 | - |
dc.contributor.AlternativeAuthor | 임윤규 | - |
dc.contributor.AlternativeAuthor | 박봉균 | - |
dc.contributor.AlternativeAuthor | 강보규 | - |
dc.contributor.AlternativeAuthor | 송대섭 | - |
dc.identifier.doi | 10.1128/CVI.13.4.520-524.2006 | - |
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