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Multiplex polymerase chain reaction detection of black pigmented bacteria in infections of endodonticigin.

Cited 20 time in Web of Science Cited 17 time in Scopus
Authors
Cho, Byung-Hoon; Seol, Jung-Hwan; Chung, Chong-Pyoung; Bae, Kwang-Shik
Issue Date
2006-02
Publisher
Elsevier
Citation
Journal of Endodontics 2006;32:110-114
Keywords
Multiplex polymerase chain reactionRapid ID 32 Ablack-pigmented bacteria
Abstract
The purpose of this study was to detect the presence of Porphyromonas endodontalis, P. gingivalis, Prevotella intermedia, P. nigrescens, and P. tannerae from clinical samples using multiplex polymerase chain reactions (PCR). Two different multiplex PCR protocols were used (one for the two Porphyromonas species and the other for the three Prevotella species), each one using a primer pair specific for each target species. The results were compared to those of the conventional culture procedures. Microbial samples were taken aseptically from 40 infected root canals and abscesses from patients. Samples were cultured in an anaerobic condition for conventional identification using a Rapid ID 32 A kit. Multiplex PCR was processed using the DNA extracted from each sample. At least one of the five species of black-pigmented bacteria (BPB) were detected in 65% (26 of 40) of the samples using multiplex PCR, and in 15% (6 of 40) using the conventional culture procedures. Multiplex PCR was more rapid, sensitive, specific, and effective in detecting BPB than the conventional culture procedures.
ISSN
0099-2399
Language
English
URI
https://hdl.handle.net/10371/66629
DOI
https://doi.org/10.1016/j.joen.2005.10.020
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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