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N-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells

DC Field Value Language
dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorJun, Ji Hae-
dc.contributor.authorLee, Sun-Hwan-
dc.contributor.authorKwak, Han Bok-
dc.contributor.authorLee, Zang Hee-
dc.contributor.authorSeo, Sang-Beum-
dc.contributor.authorWoo, Kyung Mi-
dc.contributor.authorKim, Gwan-Shik-
dc.contributor.authorBaek, Jeong-Hwa-
dc.date.accessioned2010-05-17T23:38:40Z-
dc.date.available2010-05-17T23:38:40Z-
dc.date.issued2008-
dc.identifier.citationJournal of Cellular Biochemistry 103:1246-1255en
dc.identifier.issn0730-2312-
dc.identifier.urihttps://hdl.handle.net/10371/66630-
dc.description.abstractEstrogen deficiency causes osteoporosis via increased generation of reactive oxygen species (ROS), and thus, antioxidants may prove to be the effective therapeutic candidates. We examined the effects of the antioxidant N-acetylcysteine (NAC) on osteoblastic differentiation in mouse calvarial cells. NAC (10-30 mM) enhanced alkaline phosphatase activity, mRNA expression of osteoblast differentiation-associated genes and mineralized nodule formation. It also increased expression of bone morphogenetic proteins-2, -4, and -7. The osteogenic activity of NAC was partially reduced by inhibition of glutathione synthesis. Since caffeic acid phenethyl ester did not stimulate osteoblast differentiation, it is unlikely that ROS scavenging activity of NAC is sufficient for osteogenic activity. We observed that NAC suppressed small GTPase RhoA activity and activation of RhoA by Pasteurella multocida toxin suppressed the osteogenic activity of NAC. These results suggest that NAC might exert its osteogenic activity via increased glutathione synthesis and inhibition of RhoA activation.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectN-acetylcysteineen
dc.subjectosteoblast differentiationen
dc.subjectglutathioneen
dc.subjectinhibition of RhoA activityen
dc.titleN-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor전지해-
dc.contributor.AlternativeAuthor이선환-
dc.contributor.AlternativeAuthor곽한복-
dc.contributor.AlternativeAuthor이장희-
dc.contributor.AlternativeAuthor서상범-
dc.contributor.AlternativeAuthor우경미-
dc.contributor.AlternativeAuthor김관식-
dc.contributor.AlternativeAuthor백정화-
dc.identifier.doi10.1002/jcb.21508-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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