Browse

Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization

DC Field Value Language
dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorWoo, Kyung Mi-
dc.contributor.authorJun, Ji-Hae-
dc.contributor.authorChen, Victor J-
dc.contributor.authorSeo, Jihye-
dc.contributor.authorBaek, Jeong-Hwa-
dc.contributor.authorKim, Gwan-Shik-
dc.contributor.authorSomerman, Martha J-
dc.contributor.authorMa, Peter X-
dc.date.accessioned2010-05-17T23:49:34Z-
dc.date.available2010-05-17T23:49:34Z-
dc.date.issued2007-01-
dc.identifier.citationBiomaterials 2007;28:335-343en
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/10371/66633-
dc.description.abstractNano-fibrous poly(l-lactic acid) (PLLA) scaffolds with interconnected pores were developed under the hypothesis that nano-fibrous scaffolding would mimic a morphological function of collagen fibrils to create a more favorable microenvironment for cells versus solid-walled scaffolds. In this study, an in vitro system was used to examine biological properties of the nano-fibrous scaffolds compared with those of solid-walled scaffolds for their potential use in bone tissue engineering. Biomineralization was enhanced substantially on the nano-fibous scaffolds compared to solid-walled scaffolds, and this was confirmed by von Kossa staining, measurement of calcium contents, and transmission electron microscopy. In support of this finding, osteoblasts cultured on the nano-fibrous scaffolds exhibited higher alkaline phosphatase activity and an earlier and enhanced expression of the osteoblast phenotype versus solid-walled scaffolds. Most notable were the increases in runx2 protein and in bone sialoprotein mRNA in cells cultured on nano-fibrous scaffolds versus solid-walled scaffolds. At the day 1 of culture, α2 and β1 integrins as well as αv and β3 integrins were highly expressed on the surface of cells seeded on nano-fibrous scaffolds, and linked to this were higher levels of phospho-Paxillin and phospho-FAK in cell lysates. In contrast, cells seeded on solid-walled scaffolds expressed significantly lower levels of these integrins, phospho-Paxillin, and phospho-FAK. To further examine the role of nano-fibrous architecture, we inhibited the formation of collagen fibrils by adding 3,4-dehydroproline to cultures and then assayed cells for expression of α2 integrin. Cells seeded on nano-fibrous scaffolds sustained expression of α2 integrin in the presence of dehydroproline, while suppression of α2 integrin was evident in cells seeded on solid-walled scaffolds. These results provide initial evidence that synthetic nano fibers may exhibit certain properties that are comparable to natural collagen fibers, and thus, the nano-fibrous architecture may serve as a superior scaffolding versus solid-walled architecture for promoting osteoblast differentiation and biomineralization.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectNano fiberen
dc.subjectOsteoblasten
dc.subjectPolymer scaffolden
dc.subjectBone regenerationen
dc.subjectTissue engineeringen
dc.subjectIntegrinen
dc.subjectBiomineralizationen
dc.titleNano-fibrous scaffolding promotes osteoblast differentiation and biomineralizationen
dc.typeArticleen
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor우경미-
dc.contributor.AlternativeAuthor전지해-
dc.contributor.AlternativeAuthor서지혜-
dc.contributor.AlternativeAuthor백정화-
dc.contributor.AlternativeAuthor김관식-
dc.identifier.doi10.1016/j.biomaterials.2006.06.013-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse