Publications

Detailed Information

Antifibrotic effect of silymarin in rat secondary biliary fibrosis is mediated by downregulation of procollagen α1(I) and TIMP-1

DC Field Value Language
dc.contributor.authorJia, Ji-Dong-
dc.contributor.authorBauer, Michael-
dc.contributor.authorCho, Jae-Jin-
dc.contributor.authorRuehl, Martin-
dc.contributor.authorMilani, Stefano-
dc.contributor.authorBoigk, Gabriele-
dc.contributor.authorRiecken, Ernst Otto-
dc.contributor.authorSchuppan, Detlef-
dc.date.accessioned2010-06-04T03:16:00Z-
dc.date.available2010-06-04T03:16:00Z-
dc.date.issued2001-
dc.identifier.citationJournal of Hepatology 2001;35:392-398en
dc.identifier.issn0168-8278-
dc.identifier.urihttps://hdl.handle.net/10371/67301-
dc.description.abstractBackground/Aims: Silymarin reduces hepatic collagen accumulation by 35% in rats with secondary biliary cirrhosis. The aim of the present study was to explore its antifibrotic mechanism.

Methods: Thirty female adult Wistar rats were allocated to (1) bile duct occlusion, (2) bile duct occlusion and oral silymarin at 50 mg/kg per day, and (3) sham operation and oral silymarin at 50 mg/kg per day. Steady-state mRNA levels for procollagen α1(I), tissue inhibitor of metalloproteinases-1 (TIMP-1), and transforming growth factor (TGF) β1 were determined by multi-probe ribonuclease protection assay.

Results: After 6 weeks of bile duct occlusion, liver collagen content was increased 12-fold, when compared with the sham-operated controls. These animals displayed 17-, 6.5- and 16-fold higher transcript levels for procollagen α1(I), TIMP-1 and TGFβ1 (P<0.01). Silymarin downregulated elevated procollagen α1(I), TIMP-1 and TGFβ1 mRNA levels by 40–60% (P<0.01). These lowered hepatic profibrogenic transcript levels correlated with decreased serum levels of the aminoterminal propeptide of procollagen type III.

Conclusions: Silymarin suppresses expression of profibrogenic procollagen α1(I) and TIMP-1 most likely via downregulation of TGFβ1 mRNA in rats with biliary fibrosis. The serum procollagen type III propeptide level mirrors profibrogenic mRNA expression in the liver.
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectAntifibrotic therapyen
dc.subjectCirrhosisen
dc.subjectCollagenen
dc.subjectFibrosisen
dc.subjectFibrogenesisen
dc.subjectLiveren
dc.subjectMatrix metalloproteinase-3en
dc.subjectMatrix metalloproteinase-13en
dc.subjectAminoterminal propeptide of procollagen type IIIen
dc.subjectProcollagenen
dc.titleAntifibrotic effect of silymarin in rat secondary biliary fibrosis is mediated by downregulation of procollagen α1(I) and TIMP-1en
dc.typeArticleen
dc.contributor.AlternativeAuthor조재진-
dc.identifier.doi10.1016/S0168-8278(01)00148-9-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share