Publications
Detailed Information
In vitro developmental potential of interspecies nuclear transfer embryos using bovine oocytes and mouse embryonic fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이창규 | - |
dc.contributor.author | 구본식 | - |
dc.date.accessioned | 2010-06-07T07:09:57Z | - |
dc.date.available | 2010-06-07T07:09:57Z | - |
dc.date.copyright | 2004. | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000053968 | eng |
dc.identifier.uri | https://hdl.handle.net/10371/67614 | - |
dc.description | Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004. | en |
dc.description.abstract | Development of somatic cell nuclear transfer(NT) technology to produce clone animals opens a new
era in the field of reproductive biology and medicine, and the birth of clones was reported in several species. However, the efficacy of this system still remains low and oocytes reconstructed with somatic cells had lower developmental competence than embryos derived from in vitro fertilization. In using bovine oocytes as a recipient, embryos have been obtained through NT of somatic cells from several cell types. Although recipient oocytes are not derived from the same species used for a donor, NT generates interspecies NT embryos. They might be used to find answers of some questions in basic aspect of NT. Therefore, we investigated the development potential of interspecies NT embryos using mouse embryonic fibroblasts and examine blastocysts rates, antioxidants effect and gene expression. To investigate the effect of bovine cytoplast on developmental block of interspecies NT embryos, two groups of media were used. One group is consisted of mouse culture media with normal (able to overcome developmental block) and block (induce 2-cell block). The other group is same combination with bovine culture media (CR1aa). As a results, interspecies NT embryos were not blocked at 2-cell stage in any type medium. A majority of the interspecies NT embryos showed abnormal development in bovine culture media. In order to overcome abnormality and blocking at 8-cell stage, we examine that antioxidant affected on developmental potential. There are four different Experiments. First experiment was cultured in CR1aa with 5.5μM BME and second experiment was cultured in CR1aa with 11μM BME. Each blastocysts rates were 2.2% and 3.8%, respectively. And, third experiment was cultured in CR1aa with 11μM BME plus 2mM GTH and fourth experiment was cultured in CR1aa with 1μM BME plus 100μM GTH. Each blastocyst rates were 1.1% and 3.6%, respectively. To know the mechanisms of Xist gene expression, we need to examine the exact timing of its onset. Earlier studies reported that Xist is expressed during development beginning from the 4-cell stage in mouse female preimplantation embryos. but we could not find it at the 4-cell stage interspecies embryo. The interspecies embryo did not show similarity of mouse embryo''s expression. β-actin mRNA was detected in all mouse embryo but FGF4, IGF2 receptor mRNA present in mouse 8-cell stage embryo. Expression of several gene, such as FGF4, IGF2 receptor, was only detected in normal mouse embryo and poly(A) polymerase in bovine gene was only detected in interspecies NT embryo. Thus, we think that the gene expression in interspecies NT embryo was abnormal. | en |
dc.format.extent | vi, 44 leaves | en |
dc.language.iso | en | en |
dc.publisher | 서울대학교 대학원 | en |
dc.subject | 이종간 핵이식 | en |
dc.subject | Interspecies nuclear transfer | en |
dc.subject | Block 배양액 | en |
dc.subject | Block media | en |
dc.subject | 항산화제 | en |
dc.subject | Antioxidant | en |
dc.subject | 유전자 발현 | en |
dc.subject | Gene expression | en |
dc.title | In vitro developmental potential of interspecies nuclear transfer embryos using bovine oocytes and mouse embryonic fibroblasts | en |
dc.type | Thesis | - |
dc.contributor.department | 농생명공학부 | - |
dc.description.degree | Master | en |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.