S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Analysis of soil contamination by phthalate ester around tributaries to the Han river and molecular identification of the phthalate-degrading bacteria
한강지류 하천 주변 토양의 phthalate ester 오염 분석 및 phthalate 분해균의 분자생물학적 동정
- Issue Date
- 서울대학교 대학원
- GC/MS; Phthalate esters; phthalate 분해 세균; GC/MS; 16S rDNA; Phthalate-degrading bacteria; 기질 특이성; 16s rDNA; Substrate specificity; LC/MS/MS
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- Phthalate esters are used as additives in plastics to improve mechanical properties,
particularly flexibility. The contamination of soil samples by phthalate esters around four
tributaries to the Han River was analyzed using gas chromatography/mass spectrometry (GC/MS).
All soil samples collected from the four tributaries, which include Anyangcheon and Seohocheon
(potential polluted areas), and Gapyeongcheon and Namhan River (potential clean areas), were
found to be contaminated by bis(2-ethylhexyl) phthalate (DEHP). Soil around Anyangcheon was most
highly contaminated (113 ppb), about twofold higher than the ones around Seohocheon (64 ppb),
Gapyeongcheon and Namhan River (50 ppb). Based on the fact that DEHP contamination in sediments
of the river tributaries around the country is as high as 2.04 ppm, it was concluded that DEHP
contamination of the soils around the tributaries to the Han River is relatively low.
Bacteria, using phthalates as carbon and energy sources, were isolated from soil highly
contaminated with phthalate. They were then identified through 16S ribosomal DNA (rDNA)
sequencing, and their substrate specificity was compared. Four different isolates were obtained
from the pure culture in phthalic acid (PA)- or DEHP-containing minimal media. As a result of
comparing bacterial 16S rDNA sequences with database, the isolated bacteria were found to belong
to three genera: Comamonas, Acinetobacter, and Pseudomonas. Comamonas testosteroni specifically
used PA as the carbon source, whereas Acinetobacter spp. and Pseudomonas tolaasii used PA and
DEHP at similar frequencies. These results indicate that the phthalate metabolism depends on
soil bacterial genera.
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