Publications
Detailed Information
Vitamin C suppresses proliferation of the human melanoma cell SK-MEL-2 through the inhibition of cyclooxygenase-2 (COX-2) expression and the modulation of insulin-like growth factor II (IGF-II) production
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seung Koo | - |
dc.contributor.author | Kang, Jae Seung | - |
dc.contributor.author | Jung, Da Jung | - |
dc.contributor.author | Hur, Dae Young | - |
dc.contributor.author | Kim, Jee Eun | - |
dc.contributor.author | Hahm, Eunsil | - |
dc.contributor.author | Bae, Seyeon | - |
dc.contributor.author | Kim, Hyung Woo | - |
dc.contributor.author | Kim, Daejin | - |
dc.contributor.author | Cho, Byung Joo | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Shin, Dong Hoon | - |
dc.contributor.author | Hwang, Young-Il | - |
dc.contributor.author | Lee, Wang Jae | - |
dc.date.accessioned | 2010-06-25T05:13:31Z | - |
dc.date.available | 2010-06-25T05:13:31Z | - |
dc.date.issued | 2008-02-26 | - |
dc.identifier.citation | J Cell Physiol. 216(1):180-188 | en |
dc.identifier.issn | 1097-4652 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18297687 | - |
dc.identifier.uri | https://hdl.handle.net/10371/67791 | - |
dc.description.abstract | Vitamin C plays a crucial role in the suppression of proliferation of several types of cancer. Over-expression of cyclooxygenase (COX)-2 and type I insulin-like growth factor (IGF) receptor are important for proliferation and protection from apoptosis in malignancies. However, its specific mechanisms, especially the interaction between COX-2 expression and IGF-I axis mediated by vitamin C, remain yet to be clarified. Therefore, we investigated the effects of vitamin C on the proliferation of melanoma cells via the modulation of COX-2 expression and IGF-I axis. As a result, we found that 1.0 mM vitamin C inhibits the proliferation of SK-MEL-2 without induction of apoptosis. At that moment, IGF-II production was decreased, followed by the inhibition of COX-2 activity. IGF-IR expression was also down-regulated by vitamin C treatment. It coincided with the result from the inhibition of COX-2 by NS-398 and COX-2 siRNA. In addition, the decreased IGF-IR expression by vitamin C was restored by the treatment of recombinant prostaglandin E2. Finally, we determined whether the signal pathway would be involved in vitamin C-induced IGF-II and IGF-IR down-regulation. When the cells were exposed to SB203580, a specific inhibitor of p38 MAPK, COX-2 expression was dramatically recovered. In addition, phosphorylated p38 MAPK was increased after vitamin C treatment. Taken together, vitamin C suppresses proliferation of the human melanoma cell line SK-MEL2 via the down-regulation of IGF-II production and IGF-IR expression, which is followed by the activation of p38 MAPK and the inhibition of COX-2 expression. | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Animals | en |
dc.subject | Ascorbic Acid/*metabolism | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cyclooxygenase 2/genetics/*metabolism | en |
dc.subject | Enzyme Activation | en |
dc.subject | Enzyme Inhibitors/metabolism | en |
dc.subject | Humans | en |
dc.subject | Imidazoles/metabolism | en |
dc.subject | Insulin-Like Growth Factor II/genetics/*metabolism | en |
dc.subject | Pyridines/metabolism | en |
dc.subject | RNA, Small Interfering/genetics/metabolism | en |
dc.subject | Receptor, IGF Type 1/genetics/metabolism | en |
dc.subject | Vitamins/metabolism | en |
dc.subject | p38 Mitogen-Activated Protein Kinases/genetics/metabolism | en |
dc.subject | Cell Proliferation | - |
dc.subject | Melanoma/metabolism/pathology | - |
dc.title | Vitamin C suppresses proliferation of the human melanoma cell SK-MEL-2 through the inhibition of cyclooxygenase-2 (COX-2) expression and the modulation of insulin-like growth factor II (IGF-II) production | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이승구 | - |
dc.contributor.AlternativeAuthor | 강재승 | - |
dc.contributor.AlternativeAuthor | 정대정 | - |
dc.contributor.AlternativeAuthor | 허대영 | - |
dc.contributor.AlternativeAuthor | 김지은 | - |
dc.contributor.AlternativeAuthor | 함은실 | - |
dc.contributor.AlternativeAuthor | 배세연 | - |
dc.contributor.AlternativeAuthor | 김형우 | - |
dc.contributor.AlternativeAuthor | 김대진 | - |
dc.contributor.AlternativeAuthor | 조병주 | - |
dc.contributor.AlternativeAuthor | 조대호 | - |
dc.contributor.AlternativeAuthor | 신동훈 | - |
dc.contributor.AlternativeAuthor | 황영일 | - |
dc.contributor.AlternativeAuthor | 이왕재 | - |
dc.identifier.doi | 10.1002/jcp.21391 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.