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Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner

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dc.contributor.authorKim, Hyun-Soo-
dc.contributor.authorJeon, Yoon-Kyung-
dc.contributor.authorHa, Geun-Hyoung-
dc.contributor.authorPark, Hye-Young-
dc.contributor.authorKim, Yu-Jin-
dc.contributor.authorShin, Hyun-Jin-
dc.contributor.authorLee, Chang Geun-
dc.contributor.authorChung, Doo-Hyun-
dc.contributor.authorLee, Chang-Woo-
dc.date.accessioned2010-06-30T07:37:57Z-
dc.date.available2010-06-30T07:37:57Z-
dc.date.issued2009-01-02-
dc.identifier.citationCancer Res. 2009;69(1):27-36en
dc.identifier.issn1538-7445 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19117984-
dc.identifier.urihttp://cancerres.aacrjournals.org/cgi/reprint/69/1/27.pdf-
dc.identifier.urihttps://hdl.handle.net/10371/68058-
dc.description.abstractActivation of the mitotic checkpoint requires the precise timing and spatial organization of mitotic regulatory events, and ensures accurate chromosome segregation. Mitotic checkpoint proteins such as BubR1 and Mad2 bind to Cdc20, and inhibit anaphase-promoting complex/cyclosome(Cdc20)-mediated securin degradation and the onset of anaphase. BubR1 mediates the proper attachment of microtubules to kinetochores, and links the regulation of chromosome-spindle attachment to mitotic checkpoint signaling. Therefore, disruption of BubR1 activity results in a loss of the checkpoint control, chromosome instability, and/or early onset of malignancy. In this study, we show that BubR1 directly interacts with securin in vitro and in vivo. In addition, the BubR1 interaction contributes to the stability of securin, and there is a significant positive correlation between BubR1 and securin expressions in human cancer. Importantly, BubR1 competes with Cdc20 for binding to securin, and thereby the interaction between BubR1 and securin is greatly increased by the depletion of Cdc20. Our findings may identify a novel regulation of BubR1 that can generate an additional anaphase-inhibitory signal through the Cdc20-independent interaction of BubR1 with securin.en
dc.description.sponsorshipKorea Health 21 R&D Project, Ministry of Health and Welfare
(03-PJ10-PG13-GD01-0002), the Korea Research Foundation (KRF-2006-312-C00625),
and the 21C Frontier Functional Human Genome Project from the Ministry of Science
& Technology in Korea (FG07-21-01).
en
dc.language.isoenen
dc.publisherAmerican Association for Cancer Researchen
dc.subjectBinding, Competitiveen
dc.subjectCell Cycle Proteins/*metabolismen
dc.subjectHCT116 Cellsen
dc.subjectHela Cellsen
dc.subjectHumansen
dc.subjectMitosis/physiologyen
dc.subjectNeoplasm Proteins/genetics/*metabolismen
dc.subjectProtein-Serine-Threonine Kinases/genetics/*metabolismen
dc.subjectTransfectionen
dc.subjectUbiquitin-Protein Ligase Complexes/*metabolismen
dc.titleFunctional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manneren
dc.typeArticleen
dc.contributor.AlternativeAuthor김현수-
dc.contributor.AlternativeAuthor전윤경-
dc.contributor.AlternativeAuthor하근형-
dc.contributor.AlternativeAuthor박혜경-
dc.contributor.AlternativeAuthor김유진-
dc.contributor.AlternativeAuthor신현진-
dc.contributor.AlternativeAuthor이창근-
dc.contributor.AlternativeAuthor정두현-
dc.contributor.AlternativeAuthor이창우-
dc.identifier.doi10.1158/0008-5472.CAN-08-0820-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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