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Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Joong Shin | - |
dc.contributor.author | Oh, Kyoung-Jin | - |
dc.contributor.author | Norwitz, Errol R | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.contributor.author | Choi, Hye-Jin | - |
dc.contributor.author | Seong, Hyo Suk | - |
dc.contributor.author | Kang, Yoon Dan | - |
dc.contributor.author | Park, Chan-Wook | - |
dc.contributor.author | Kim, Byoung Jae | - |
dc.contributor.author | Jun, Jong Kwan | - |
dc.contributor.author | Syn, Hee Chul | - |
dc.date.accessioned | 2010-07-01T03:34:40Z | - |
dc.date.available | 2010-07-01T03:34:40Z | - |
dc.date.issued | 2008-06-27 | - |
dc.identifier.citation | Reprod Sci. 2008;15(5):457-468 | en |
dc.identifier.issn | 1933-7205 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18579854 | - |
dc.identifier.uri | http://rsx.sagepub.com/cgi/reprint/15/5/457.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68095 | - |
dc.description.abstract | OBJECTIVE: To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR). METHODS: AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR. CONCLUSION: Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42. | en |
dc.language.iso | en | en |
dc.publisher | SAGE Publications | en |
dc.subject | Adult | en |
dc.subject | Amniotic Fluid/*chemistry/metabolism | en |
dc.subject | Apolipoprotein A-I/*analysis | en |
dc.subject | Biological Markers/analysis | en |
dc.subject | Diagnosis, Differential | en |
dc.subject | Female | en |
dc.subject | Humans | en |
dc.subject | Hypertension/diagnosis/metabolism | en |
dc.subject | Infant, Newborn | en |
dc.subject | Pilot Projects | en |
dc.subject | Pre-Eclampsia/diagnosis/*metabolism | en |
dc.subject | Pregnancy | en |
dc.subject | Proteomics/*methods | en |
dc.subject | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods | en |
dc.title | Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박중신 | - |
dc.contributor.AlternativeAuthor | 오경진 | - |
dc.contributor.AlternativeAuthor | 한중수 | - |
dc.contributor.AlternativeAuthor | 최혜진 | - |
dc.contributor.AlternativeAuthor | 성효석 | - |
dc.contributor.AlternativeAuthor | 강윤단 | - |
dc.contributor.AlternativeAuthor | 박찬욱 | - |
dc.contributor.AlternativeAuthor | 김병재 | - |
dc.contributor.AlternativeAuthor | 전종관 | - |
dc.contributor.AlternativeAuthor | 신희철 | - |
dc.identifier.doi | 10.1177/1933719108316909 | - |
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