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Activation of PPARgamma negatively regulates O-GlcNAcylation of Sp1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Sung Soo | - |
dc.contributor.author | Kim, Ji Hyun | - |
dc.contributor.author | Park, Ho Seon | - |
dc.contributor.author | Choi, Hye Hun | - |
dc.contributor.author | Lee, Kyeong Won | - |
dc.contributor.author | Cho, Young Min | - |
dc.contributor.author | Lee, Hong Kyu | - |
dc.contributor.author | Park, Kyong Soo | - |
dc.date.accessioned | 2010-07-01T23:28:43Z | - |
dc.date.available | 2010-07-01T23:28:43Z | - |
dc.date.issued | 2008-06-03 | - |
dc.identifier.citation | Biochem Biophys Res Commun. 372 (2008) 713–718 | en |
dc.identifier.issn | 1090-2104 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18513490 | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4SM6R6T-1-9&_cdi=6713&_user=168665&_orig=search&_coverDate=08%2F08%2F2008&_sk=996279995&view=c&wchp=dGLzVtb-zSkWb&md5=5c07d07cb563206772d0313dd3c41a0f&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68184 | - |
dc.description.abstract | O-GlcNAcylation is a kind of post-translational modification and many nuclear and cytoplasmic proteins are O-GlcNAcylated. In this study, we demonstrated that thiazolidinediones (TZDs), which are used as insulin sensitizer, specifically inhibited the O-GlcNAcylation of Sp1 but did not affect the O-GlcNAcylation of the total proteins in cell culture systems and mouse models. This effect was mediated by peroxisome proliferator activated receptor gamma (PPARgamma) activation and probably by synthesis of a specific protein induced by PPARgamma activation. In addition, we demonstrated that the O-GlcNAcylation sites in the zinc-finger domain were involved in the transcriptional activation of Sp1 and that rosiglitazone, a member of TZDs, affected Sp1 transcriptional activity partially by regulating the O-GlcNAcylation level of these sites. Considering the role of hexosamine biosynthesis pathway in hyperglycemia-induced insulin resistance and Sp1 in the hyperglycemia-induced gene expression, the regulation of Sp1 O-GlcNAcylation by TZDs may help to explain the function of TZDs as a treatment for insulin resistance and diabetes. | en |
dc.description.sponsorship | This study was supported by a grant from the Innovative Research
Institute for Cell Therapy, Republic of Korea (Project No. A062260) and a grant from Marine Biotechnology Program funded by Ministry of Land, Transport, and Maritime Affairs, Republic of Korea. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Acetylglucosamine/biosynthesis/*metabolism | en |
dc.subject | Acylation/drug effects | en |
dc.subject | Animals | en |
dc.subject | Cell Line | en |
dc.subject | Humans | en |
dc.subject | Hyperglycemia/metabolism | en |
dc.subject | Hypoglycemic Agents/*pharmacology | en |
dc.subject | Insulin Resistance | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | PPAR gamma/*agonists | en |
dc.subject | Protein Processing, Post-Translational/*drug effects | en |
dc.subject | Sp1 Transcription Factor/*metabolism | en |
dc.subject | Thiazolidinediones/*pharmacology | en |
dc.subject | Transcription, Genetic | en |
dc.subject | Zinc Fingers | en |
dc.title | Activation of PPARgamma negatively regulates O-GlcNAcylation of Sp1 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정성수 | - |
dc.contributor.AlternativeAuthor | 김지현 | - |
dc.contributor.AlternativeAuthor | 박호선 | - |
dc.contributor.AlternativeAuthor | 최혜훈 | - |
dc.contributor.AlternativeAuthor | 이경원 | - |
dc.contributor.AlternativeAuthor | 조영민 | - |
dc.contributor.AlternativeAuthor | 이홍규 | - |
dc.contributor.AlternativeAuthor | 박경수 | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.05.096 | - |
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