Browse

Pre-treatment of mesenchymal stem cells with a combination of growth factors enhances gap junction formation, cytoprotective effect on cardiomyocytes, and therapeutic efficacy for myocardial infarction

DC Field Value Language
dc.contributor.authorHahn, Joo-Yong-
dc.contributor.authorCho, Hyun-Ju-
dc.contributor.authorKang, Hyun-Jae-
dc.contributor.authorKim, Tack-Seung-
dc.contributor.authorKim, Mi-Hyung-
dc.contributor.authorChung, Jung-Hwa-
dc.contributor.authorBae, Jang-Whan-
dc.contributor.authorOh, Byung-Hee-
dc.contributor.authorPark, Young-Bae-
dc.contributor.authorKim, Hyo-Soo-
dc.date.accessioned2010-07-02T04:13:28Z-
dc.date.available2010-07-02T04:13:28Z-
dc.date.issued2008-03-01-
dc.identifier.citationJ Am Coll Cardiol. 2008;51(9):933-943en
dc.identifier.issn1558-3597 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18308163-
dc.identifier.urihttp://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T18-4RXN2M5-B-8&_cdi=4884&_user=168665&_orig=search&_coverDate=03%2F04%2F2008&_sk=999489990&view=c&wchp=dGLbVlW-zSkzk&md5=133bf0cd261f4fdb667bdb807e1e7b62&ie=/sdarticle.pdf-
dc.identifier.urihttps://hdl.handle.net/10371/68189-
dc.description.abstractOBJECTIVES: The goal of this study was to investigate the effect of pre-treatment of mesenchymal stem cells (MSCs) with growth factors (GFs) on cardiomyogenic differentiation, cytoprotective action on cardiomyocytes (CMCs), and their therapeutic efficacy in myocardial infarction. BACKGROUND: Mechanisms of myocardial repair with MSC transplantation have not been fully elucidated, and therapeutic efficacy needs to be enhanced. METHODS: The MSCs obtained from the bone marrow of Fisher344 rats were treated with fibroblast growth factor-2, insulin-like growth factor-1, and bone morphogenetic protein-2. The expression of cardiac specific markers and the cytoprotective effect of MSCs with its mechanism were evaluated. Efficacy of MSCs transplantation was studied in rat myocardial infarction model. RESULTS: Treatment of MSCs with cocktails of GFs enhanced expression of cardiac transcription factors and survival. Induction of cardiac specific markers by coculture with CMCs and gap junctional communication with CMCs was more active in GF-treated MSCs than untreated MSCs. The GF-treated MSCs reduced apoptosis of neighboring CMCs in a hypoxic condition and enhanced the phosphorylated Akt and phosphorylated c-AMP response element binding protein expression of CMCs, which was markedly reduced by gap junction blockade. In a rat myocardial infarction model, transplantation of GF-treated MSCs resulted in smaller infarct size and better cardiac function than transplantation of untreated MSCs. Additionally, GF treatment enhanced gap junction formation of transplanted MSCs, which did not aggravate arrhythmia. CONCLUSIONS: Pre-treatment of MSCs with GFs enhanced cytoprotective effects on neighboring CMCs through gap junction and improved the therapeutic efficacy of MSC transplantation for myocardial repair. "Priming of MSCs with GFs" before transplantation might improve the therapeutic efficacy of cell therapy.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectAnimalsen
dc.subjectBone Morphogenetic Protein 2en
dc.subjectBone Morphogenetic Proteins/*pharmacologyen
dc.subjectCell Differentiation/drug effectsen
dc.subjectDisease Models, Animalen
dc.subjectFibroblast Growth Factor 2/*pharmacologyen
dc.subjectGap Junctions/*drug effects/metabolismen
dc.subjectGene Expressionen
dc.subjectInsulin-Like Growth Factor I/*pharmacologyen
dc.subjectMaleen
dc.subjectMesenchymal Stem Cell Transplantationen
dc.subjectMesenchymal Stem Cells/*drug effectsen
dc.subjectMyocardial Infarction/*therapyen
dc.subjectMyocytes, Cardiac/*drug effects/metabolismen
dc.subjectRatsen
dc.subjectRats, Inbred F344en
dc.subjectTransforming Growth Factor beta/*pharmacologyen
dc.titlePre-treatment of mesenchymal stem cells with a combination of growth factors enhances gap junction formation, cytoprotective effect on cardiomyocytes, and therapeutic efficacy for myocardial infarctionen
dc.typeArticleen
dc.contributor.AlternativeAuthor한주용-
dc.contributor.AlternativeAuthor조현주-
dc.contributor.AlternativeAuthor강현재-
dc.contributor.AlternativeAuthor김택승-
dc.contributor.AlternativeAuthor김미형-
dc.contributor.AlternativeAuthor정정화-
dc.contributor.AlternativeAuthor배장환-
dc.contributor.AlternativeAuthor오병희-
dc.contributor.AlternativeAuthor박영배-
dc.contributor.AlternativeAuthor김효수-
dc.identifier.doi10.1016/j.jacc.2007.11.040-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse