S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Neurology (신경과학교실) Journal Papers (저널논문_신경과학교실)
Autophagy is involved in the ischemic preconditioning
- Park, Hee-Kwon; Chu, Kon; Jung, Keun-Hwa; Lee, Soon-Tae; Bahn, Jae-Joon; Kim, Manho; Lee, Sang Kun; Roh, Jae-Kyu
- Issue Date
- Neurosci Lett. 451 (2009) 16-19
- Adenine/analogs & derivatives/pharmacology; Animals; Autophagy/*physiology; Cathepsin D/metabolism; Cell Survival/drug effects/physiology; HSP70 Heat-Shock Proteins/metabolism; Hypoxia-Ischemia, Brain/*metabolism/physiopathology; Immunohistochemistry; *Ischemic Preconditioning; Lysosomes/metabolism/pathology/ultrastructure; Microscopy, Electron, Transmission; Nerve Degeneration/metabolism/physiopathology; Neurons/*metabolism/pathology/ultrastructure; PC12 Cells; Phagosomes/*metabolism/pathology/ultrastructure; Rats; Reperfusion Injury/metabolism/physiopathology; Tetrazolium Salts
- Autophagy is a key pathway for the clearance of damaged organelles. Ischemic preconditioning (IPC) and autophagy are enhanced by mild hypoxic insults, but the association between autophagy and IPC remains unclear. We investigated the existence and role of autophagy in IPC. In an in vitro PC12 cell model, IPC increased generation and degradation of autophagosomes, as revealed by increased LC3-II bands, cathepsin D positive cells, lysosomal activity and autophagic vacuoles on electron microscopy. Autophagic activity was blocked using 3-methyladenine during IPC, and cell viabilities were measured using FASC and WST-1 assays. Inhibition of autophagy, especially during reperfusion or lethal oxygen-glucose deprivation periods ameliorated the neuroprotective effects of IPC. Moreover, inhibiting autophagy also attenuated Hsp70 upregulation induced by IPC. These findings imply that autophagy participates in IPC-induced neuroprotection, and that autophagy might provide a means of neuroprotection against cerebral ischemia.
- 0304-3940 (Print)
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