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Gene expression patterns in glucose-stimulated podocytes
Cited 16 time in
Web of Science
Cited 19 time in Scopus
- Authors
- Issue Date
- 2008-04-09
- Publisher
- Elsevier
- Citation
- Biochem Biophys Res Commun. 370 (2008) 514-518
- Keywords
- Animals ; Blotting, Western ; Cells, Cultured ; Diabetic Nephropathies/*genetics/pathology ; Disease Models, Animal ; Gene Expression/drug effects ; Glucose/*metabolism/pharmacology ; Mice ; Podocytes/drug effects/*metabolism/pathology ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Gene Expression Profiling
- Abstract
- To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.
- ISSN
- 1090-2104 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18395008
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4S6GP8M-9-D&_cdi=6713&_user=168665&_orig=search&_coverDate=06%2F06%2F2008&_sk=996299996&view=c&wchp=dGLbVzW-zSkWA&md5=8989f8009a4144d0b3ac3f83c4e948a0&ie=/sdarticle.pdf
https://hdl.handle.net/10371/68492
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