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Development of enzyme immobilization methods by covalent binding using chitosan supports and sol-gel technology
키토산 비드를 이용한 공유결합법과 솔-젤법을 이용한 효소 고정화 방법의 개발

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Authors
이장원
Advisor
김병기
Issue Date
2003
Publisher
서울대학교 대학원
Keywords
솔-젤Sol-gel비드Bead효소 고정화Enzyme immobilization
Description
Thesis (master`s)--서울대학교 대학원 :응용화학부,2003.
Abstract
We immobilized enzyme onto chitosan beads after treating them by
glutaraldehyde to form aldehyde group as a cross-linker. We studied the optimum
conditions for immobilizing ω-Transaminase onto the chitosan beads and under
these conditions, the investigation to improve operational stability was also
performed. During the immobilizing procedure, the pH value plays a crucial role
on the efficiency of immobilization and the activity. The optimum value of pH for
immobilization is 5.5, almost similar digit to the enzyme's pI value. The efficiency of
immobilization decreased sharply beyond the optimum region. By adding the
pyridoxal 5-phosphate (PLP), the cofactor of ω-Transaminase, into the substrate
solution and by skirting the substrate inhibition, we could see a great improvement
of the operational stability. The remained activity after 5 times reuse improved to
90% from 16% of initial value.
We also could prepare sol-gel beads for enzyme encapsulation. Our experiments
mainly focused on the procedure to produce the sol-gel bead more uniformly in
shape. By controlling the solvents and sol conditions, we can get sphere shaped solgel
beads with relatively small in size.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000059130

https://hdl.handle.net/10371/68545
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Theses (Master's Degree_화학생물공학부)
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