Oligonucleotide microarray analysis of ameloblastoma compared with dentigerous cyst.

Abstract

Background: Ameloblastoma is a benign, but locally invasive tumor known for its high rate of recurrence. However, few comprehensive genetic studies have been conducted about its tumorigenesis. Our aim was to identify possible genes involved in the development and progression of ameloblastoma, using microarray analysis with dentigerous cyst as a control.

Methods: Total RNA was extracted from two fresh dentigerous cysts and ameloblastoma specimens. Following microarray analysis, semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) and immunohistochemistry were performed on selected genes.

Results: Seventy-three genes were overexpressed and 49 were underexpressed. These genes were divided into categories according to function. The microarray results for 13 selected genes were verified with semiquantitative RT–PCR.

Conclusions: We identified important genes related to the development and progression of ameloblastoma through a large-scale gene expression analysis. This study will stimulate further investigations on genes significant for early diagnosis and prognosis of ameloblastoma.