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Cell behavior of human papillomavirus-immortalized and tumorigenic human oral keratinocytes does not depend on the integrin expression

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dc.contributor.authorPark, Kyung Hee-
dc.contributor.authorMin, Byung-Moo-
dc.date.accessioned2010-07-23T05:40:39Z-
dc.date.available2010-07-23T05:40:39Z-
dc.date.issued2007-09-
dc.identifier.citationInternational journal of oral biology 32(3):93-101en
dc.identifier.issn1226-7155-
dc.identifier.urihttps://hdl.handle.net/10371/68695-
dc.description.abstractCell behavior of the transformed cells is known to affect by interaction with extracellular matrix (ECM) proteins and integrin. To investigate the alterations of both integrin expression and cell-matrix interaction during neoplastic conversion of human oral kerationcytes, we studied expression levels of integrin subunits by flow cytometry and cellular responses to the ECM proteins in normal human oral keratinocytes (NHOKs), HPV-immortalized HOK-16B line, and three oral cancer cell lines established from HOK-16B line, CTHOK-16B-BaP, CTHOK-16B-DMBA, and CTHOK-16B-Dexa lines. The expression levels of integrin subunits were shown decreased tendency in human oral keratinocytes undergoing immortalization and tumorigenic transformation except CTHOK-16B-DMBA line tested. Although integrin is known to be highly expressed in squamous cell carcinomas, and the altered integrin expression is suspected to be associated with cellular carcinogenesis, integrin subunit and integrin did not express in oral cancer cell lines tested. Cell behavior to the ECM proteins in HOK-16B line was generally similar to that of exponentially proliferating NHOKs. The adhesion activity profiles of type I collagen were very similar to that of its laminin counterparts, but fibronectin showed minimal adhesion activity under our conditions compared to the BSA control. The ability of the CTHOK-16B-BaP line to spread upon type I collagen and laminin markedly decreased, but migration was notably increased on type I collagen. In contrast, CTHOK-16B-DMBA and CTHOK-16B-Dexa lines spread less but migrated more upon type I collagen than immortalized HOK-16B line. These data indicate that downregulation of integrin subunits causes the changes of cellular responses to the ECM proteins during neoplastic conversion of human oral keratinocytes, and that cellular responses to the ECM proteins in oral cancer cell lines established by exposing different carcinogens are variable according to chemical carcinogens treatment.en
dc.description.sponsorshipThis work was supported by a grant from the KOSEF through the Intellectual Biointerface Engineering Center at Seoul National University(to B.-M. M.).en
dc.language.isoenen
dc.publisherKorean Academy of Oral Biologyen
dc.subjectCell behavioren
dc.subjectIntegrinen
dc.subjectExtracellular matrix proteinsen
dc.subjectOral cancer cellsen
dc.titleCell behavior of human papillomavirus-immortalized and tumorigenic human oral keratinocytes does not depend on the integrin expressionen
dc.typeArticleen
dc.contributor.AlternativeAuthor박경희-
dc.contributor.AlternativeAuthor민병무-
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