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Development of a chemically defined in vitro maturation system for porcine oocytes: application for somatic cell nuclear transfer

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dc.contributor.authorKoo, Ja-Min-
dc.contributor.authorWon, Cheol-Hee-
dc.contributor.authorMin, Byung-Moo-
dc.contributor.authorRoh, Sang-Ho-
dc.date.accessioned2010-08-02-
dc.date.available2010-08-02-
dc.date.issued2005-
dc.identifier.citationInternational journal of oral biology 30(4):131-134en
dc.identifier.issn1226-7155-
dc.identifier.urihttps://hdl.handle.net/10371/68821-
dc.description.abstractIn the present study, performances of several in vitro maturation (IVM) systems for porcine follicular oocytes were evaluated, and an efficient chemically defined IVM system for porcine oocytes was proposed. The proposed one-step culture system supplemented with polyvinylalcohol (PVA) gave competitive efficiencies in terms of oocyte maturation and blastocyst development after parthenogenetic activation and in vitro culture, compared with the conventional two-step culture system by a supplementation of porcine follicular fluid (pFF). Additionally, it is identified that the proposed chemically defined one-step culture system yielded the comparable level of blastocyst production to the conventional maturation system in porcine somatic cell nuclear transfer (SCNT). Therefore, one can eliminate un-expected effects accompanied by supplementation of pFF. No medium replacement during whole maturation period is an additional benefit by applying this new system. Thus, these data support that the developed PVA supplemented chemically defined one-step IVM system for porcine follicular oocyte might be used in porcine SCNT program.en
dc.description.sponsorshipThis study was supported by Technology Development Program (High-Technology Development Project, 204117-03-2-SB010) for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.en
dc.language.isoenen
dc.publisherKorean Academy of Oral Biologyen
dc.subjectIn vitro maturationen
dc.subjectporcineen
dc.subjectparthenogenesisen
dc.subjectnuclear transferen
dc.titleDevelopment of a chemically defined in vitro maturation system for porcine oocytes: application for somatic cell nuclear transferen
dc.typeArticleen
dc.contributor.AlternativeAuthor구자민-
dc.contributor.AlternativeAuthor원철희-
dc.contributor.AlternativeAuthor민병무-
dc.contributor.AlternativeAuthor노상호-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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