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Expression of Na+/HCO3- cotransporter and its role in pH regulation in mouse parotid acinar cells

Cited 19 time in Web of Science Cited 18 time in Scopus
Authors
Park, Kyungpyo; Kim, Younbae; Yang, Byung Ho; Piao, Zhang Gen; Oh, Seog Bae; Kim, Joong Soo
Issue Date
2003-05
Publisher
Elsevier
Citation
Biochemical and Biophysical Research Communications 304 (2003) 593-598
Keywords
Na+/Hco3-contransporterintracellular pHparotidEIPADIDS
Abstract
Ion transporters such as Na+/H+ exchanger (NHE), Cl−/HCO3− exchanger (AE), and Na+/HCO3− cotransporter (NBC) are known to contribute to the intracellular pH (pHi) regulation during agonist-induced stimulation. This study examined the mechanisms for the pHi regulation in the mouse parotid and sublingual acinar cells using the fluorescent pH-sensitive probe, BCECF. The pHi recovery from agonist-induced acidification in the sublingual acinar cells was completely blocked by EIPA, a NHE inhibitor. However, the parotid acinar cells required DIDS, a NBC1 inhibitor, in addition to EIPA in order to block the pHi recovery. Moreover, RT-PCR analysis detected the expression of pancreatic NBC1 (pNBC1) only in the parotid acinar cells. These results provide strong evidence that the mechanisms for the pHi regulation are different in the two types of acinar cells, and pNBC1 contributes to pHi regulation in the parotid acinar cells, whereas NHE is likely to be the exclusive pHi regulator in the sublingual acinar cells.
ISSN
0006-291X
Language
English
URI
https://hdl.handle.net/10371/68827
DOI
https://doi.org/10.1016/S0006-291x(03)00632-6
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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