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Bupivacaine induces apoptosis via ROS in the schwann cell line

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dc.contributor.authorPark, C.J.-
dc.contributor.authorPark, S.A.-
dc.contributor.authorYoon, T.G.-
dc.contributor.authorLee, S.J.-
dc.contributor.authorYum, K.W.-
dc.contributor.authorKim, H.J.-
dc.date.accessioned2010-08-04T01:11:58Z-
dc.date.available2010-08-04T01:11:58Z-
dc.date.issued2005-
dc.identifier.citationJ Dent Res 84(9):852-857en
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10371/68917-
dc.description.abstractLocal anesthetics have been generally accepted as being safe. However, recent clinical trials and basic studies have provided strong evidence for the neurotoxicity of local anesthetics, especially through apoptosis. We hypothesized that local anesthetics cause neural complications through Schwann cell apoptosis. Among local anesthetics tested on the Schwann cell line, RT4-D6P2T, bupivacaine significantly induced cell death, measured by the methyl tetrazolium (MTT) assay, in a dose- (LD50 = 476 µM) and time-dependent manner. The bupivacaine-induced generation of reactive oxygen species (ROS), which was initiated within 5 hrs and preceded the activation of caspase-3 and poly ADP-ribose polymerase (PARP) degradation, was suggested to trigger apoptosis, exhibited by Hoechst 33258 nuclear staining and DNA fragmentation. Furthermore, concomitant block of ROS by anti-oxidants significantly inhibited bupivacaine-induced apoptosis. Among the local anesthetics for peripheral neural blocks, bupivacaine induced apoptosis in the Schwann cell line, which may be associated with ROS production.en
dc.description.sponsorshipWe thank Se-Ra Sung for expert technical assistance. This
study was supported by the General Fund of Seoul National
University Hospital, Republic of Korea. This paper is based on
a dissertation submitted to the graduate faculty, Seoul National
University, in partial fulfillment of the requirements for the
PhD degree.
en
dc.language.isoenen
dc.publisherAmerican and International Associations for Dentalen
dc.subjectapoptosisen
dc.subjectbupivacaineen
dc.subjectneurotoxicityen
dc.subjectreactive oxygen speciesen
dc.subjectSchwann cellen
dc.titleBupivacaine induces apoptosis via ROS in the schwann cell lineen
dc.typeArticleen
dc.identifier.doi10.1177/154405910508400914-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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