Publications
Detailed Information
Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface
Cited 22 time in
Web of Science
Cited 23 time in Scopus
- Authors
- Issue Date
- 2005-10
- Publisher
- Wiley-Blackwell
- Citation
- Clin Oral Implants Res. 16(5):557-562
- Keywords
- cell attachment ; cell proliferation ; fibronectin ; magnetic field ; titanium
- Abstract
- Objectives: We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed.
Material and methods: Various magnetic fields – 1, 2, 3, 5, 7, 10 mT – were established by controlling the distance from Nd–Fe–B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 μM hFNIII9-10 at 37°C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope.
Results: The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).
Conclusion: Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
- ISSN
- 0905-7161
- Language
- English
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.