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Potentiation of PGE(2)-mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells.

DC Field Value Language
dc.contributor.authorChoi, Se-Young-
dc.contributor.authorChoi, Bo-Hwa-
dc.contributor.authorSuh, Byung-Chang-
dc.contributor.authorChae, Hee-Don-
dc.contributor.authorKim, Jong-So-
dc.contributor.authorShin, Min-Jung-
dc.contributor.authorKang, Shin-Sung-
dc.contributor.authorNegishi, Manabu-
dc.contributor.authorKim, Kyong-Tai-
dc.date.accessioned2010-08-04T23:02:41Z-
dc.date.available2010-08-04T23:02:41Z-
dc.date.issued2008-07-
dc.identifier.citationJournal of Neurochemistry. 79, 303-310en
dc.identifier.issn0022-3042-
dc.identifier.urihttps://hdl.handle.net/10371/68970-
dc.description.abstractThe prostaglandin-evoked cAMP production was studied in human neuroblastoma SK-N-BE(2)C cells during neuronal differentiation induced by all-trans retinoic acid. The incubation with 5 µm all-trans retinoic acid for 4–6 days promoted neurite outgrowth of cells. After differentiation, prostaglandin E2 (PGE2)-induced cAMP production was dramatically increased, whereas forskolin- and AlF -induced cAMP productions were not changed. The increase reached maximum after 4-days of incubation with all-trans retinoic acid. The differentiation caused an increase in the maximal response and a decrease in the half-maximal effective concentration of the PGE2-induced cAMP production. In addition, the binding of [3H]PGE2 to membrane receptors was enhanced in differentiated cells. However, the order of potency of the various prostaglandins (PGE1 = PGE2 > PGD2 = PGF2α = PGI2) in cAMP production did not change during the differentiation, suggesting that mainly E-prostanoid (EP) receptors were involved. Butaprost, an EP2 receptor specific agonist, increased the cAMP level in a concentration dependent manner and had a similar potentiating effect on cAMP production as PGE2 upon differentiation. Northern blot analysis using the human cDNA probes shows that the EP2 mRNA level was about seven times higher in differentiated cells, while the dopamine β-hydroxylase (DBH) mRNA completely disappeared. Our results, thus, suggest that elevated gene expression of the prostanoid EP2 receptor results in an increase in the PGE2-evoked cAMP production in SK-N-BE(2)C cells during neuronal differentiation.en
dc.description.sponsorshipThis work was supported by the grants of the Brain
Neurobiology Research Program, and National Research
Laboratory fund sponsored by the Ministry of Science and
Engineering and the KOSEF (2000). This work was also
supported by Brain Korea 21 program of the Ministry of
Education.
en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectcAMPen
dc.subjectEP receptoren
dc.subjectneuroblastoma cellsen
dc.subjectneuronal differentiationen
dc.subjectprostaglandinen
dc.subjecttranscriptional regulationen
dc.titlePotentiation of PGE(2)-mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells.en
dc.typeArticleen
dc.contributor.AlternativeAuthor최세영-
dc.contributor.AlternativeAuthor최보화-
dc.contributor.AlternativeAuthor서병창-
dc.contributor.AlternativeAuthor채희돈-
dc.contributor.AlternativeAuthor김종수-
dc.contributor.AlternativeAuthor신민정-
dc.contributor.AlternativeAuthor강신성-
dc.contributor.AlternativeAuthor김경태-
dc.identifier.doi10.1111/j.1471-4159.2001.00577.x-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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