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Serum replacement with a growth factor–free synthetic substance in culture medium contributes to effective establishment of mouse embryonic stem cells of various origins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seung Tae | - |
dc.contributor.author | Oh, Se Woong | - |
dc.contributor.author | Kim, Dae-Yong | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Moon, Shin Yong | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.date.accessioned | 2009-08-11T03:28:57Z | - |
dc.date.available | 2009-08-11T03:28:57Z | - |
dc.date.issued | 2006-09-06 | - |
dc.identifier.citation | Fertil Steril 2006;86:1137-45 | en |
dc.identifier.issn | 0362-028X | - |
dc.identifier.uri | https://hdl.handle.net/10371/6899 | - |
dc.description.abstract | Objective: To evaluate whether serum replacement with growth factor–free synthetic substances contributed to
the effective establishment of embryonic stem (ES) cells. Design: Randomized, prospective model study. Setting: Gamete and stem cell biotechnology laboratory at Seoul National University in Korea. Animal(s): F1 (C57BL6 DBA2) mice. Intervention(s): Blastocysts of different origins were cultured in serum-replaced media. Main Outcome Measure(s): Embryonic stem cell establishment. Result(s): Eight batches of ES cells were established from colony-forming inner cell mass cells after the replacement of fetal bovine serum (FBS) with synthetic knockout serum replacement (KSR) in mkDMEM. The established cells were positive for ES cell markers and formed both embryoid bodies in vitro and teratomas in vivo, but the established cell batches and control (transformed) ES cells responded differently to the culture media. Higher levels of cell viability were detected after the replacement with the 75:25 FBS–KSR mixture than with any other mixtures, and a gradual decrease in viability was detected as the KSR volume ratio was increased. The 75:25 FBS–KSR mixture-containing medium supported ES cell establishment of outbred ICR, F1, and F2 of C57BL6/DBA2; F1 parthenogenetic and ES cell–complemented tetraploid blastocysts; and single ES-cell cultures. Conclusion(s): A serum-replaced medium could be used for effective ES-cell establishment of various origins. (Fertil Steril 2006;86(Suppl 3):1137– 45. ©2006 by American Society for Reproductive Medicine.) | en |
dc.description.abstract | Objective: To evaluate whether serum replacement with growth factor–free synthetic substances contributed to
the effective establishment of embryonic stem (ES) cells. Design: Randomized, prospective model study. Setting: Gamete and stem cell biotechnology laboratory at Seoul National University in Korea. Animal(s): F1 (C57BL6 DBA2) mice. Intervention(s): Blastocysts of different origins were cultured in serum-replaced media. Main Outcome Measure(s): Embryonic stem cell establishment. Result(s): Eight batches of ES cells were established from colony-forming inner cell mass cells after the replacement of fetal bovine serum (FBS) with synthetic knockout serum replacement (KSR) in mkDMEM. The established cells were positive for ES cell markers and formed both embryoid bodies in vitro and teratomas in vivo, but the established cell batches and control (transformed) ES cells responded differently to the culture media. Higher levels of cell viability were detected after the replacement with the 75:25 FBS–KSR mixture than with any other mixtures, and a gradual decrease in viability was detected as the KSR volume ratio was increased. The 75:25 FBS–KSR mixture-containing medium supported ES cell establishment of outbred ICR, F1, and F2 of C57BL6/DBA2; F1 parthenogenetic and ES cell–complemented tetraploid blastocysts; and single ES-cell cultures. Conclusion(s): A serum-replaced medium could be used for effective ES-cell establishment of various origins. (Fertil Steril 2006;86(Suppl 3):1137– 45. ©2006 by American Society for Reproductive Medicine.) | en |
dc.description.sponsorship | Supported by a grant from the Stem Cell Research Center of the 21st
Century Frontier Research Program funded by the Ministry of Science and Technology, Gwacheon, Korea (SC-2170). The authors also acknowledge a graduate fellowship provided by the Korean Ministry of Education through the Brain Korea 21 project, Seoul, Korea. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Mouse | en |
dc.subject | embryonic stem (ES) cell | en |
dc.subject | establishment | en |
dc.subject | serum replacement | en |
dc.subject | single-cell culture | en |
dc.title | Serum replacement with a growth factor–free synthetic substance in culture medium contributes to effective establishment of mouse embryonic stem cells of various origins | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이승태 | - |
dc.contributor.AlternativeAuthor | 이승태 | - |
dc.contributor.AlternativeAuthor | 오세웅 | - |
dc.contributor.AlternativeAuthor | 오세웅 | - |
dc.contributor.AlternativeAuthor | 김대용 | - |
dc.contributor.AlternativeAuthor | 김대용 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 문신용 | - |
dc.contributor.AlternativeAuthor | 문신용 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.identifier.doi | 10.1016/j.fertnstert.2006.01.056 | - |
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